. "515839" . . . . "Srovn\u00E1n\u00ED komer\u010Dn\u011B dostupn\u00FDch protil\u00E1tek pro detekci centr\u00E1ln\u00ED nervov\u00E9 tk\u00E1n\u011B v masn\u00FDch v\u00FDrobc\u00EDch metodou ELISA"@cs . . "RIV/00027162:_____/05:06000015!RIV06-MZE-00027162" . "68" . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "Srovn\u00E1n\u00ED komer\u010Dn\u011B dostupn\u00FDch protil\u00E1tek pro detekci centr\u00E1ln\u00ED nervov\u00E9 tk\u00E1n\u011B v masn\u00FDch v\u00FDrobc\u00EDch metodou ELISA"@cs . . "0362-028X" . . "3" . . "Comparison of commercially available antibodies for the detection of central nervous system tissue in meat products by enzyme-linked immunosorbent assay"@en . "The utilization of four types of commercially available antibodies for the identification of the CNS tissue was tested by ELISA method. The sensitivity of the monoclonal antibodies anti-GFAP (ICN) was even 25% for both the appropriate (GFAP) antigen andthe brain tissue or spinal cord. There was no reaction concerning skeletal muscle, diaphragm or peripheral nerves. The sensitivity of the monoclonal anti-neurofilaments 200 (ICN) antibodies was also 25% for the appropriate neurofilament 200 antigen andbrain and spinal cord tissue. The sensitivity of polyclonal anti-neurofilament 200 antibodies (Sigma) was 2%. The monoclonal anti-MBP antibodies (Sigma) gave the same results and moreover the positive reaction with the peripheral nervous tissue and thesensitivity was 10%. All tested commercially available antibodies in our laboratory showed unsuitable sensitivity for the purpose of the semiquantitative identification of CNS tissue." . "Ren\u010Dov\u00E1, Eva" . "The utilization of four types of commercially available antibodies for the identification of the CNS tissue was tested by ELISA method. The sensitivity of the monoclonal antibodies anti-GFAP (ICN) was even 25% for both the appropriate (GFAP) antigen andthe brain tissue or spinal cord. There was no reaction concerning skeletal muscle, diaphragm or peripheral nerves. The sensitivity of the monoclonal anti-neurofilaments 200 (ICN) antibodies was also 25% for the appropriate neurofilament 200 antigen andbrain and spinal cord tissue. The sensitivity of polyclonal anti-neurofilament 200 antibodies (Sigma) was 2%. The monoclonal anti-MBP antibodies (Sigma) gave the same results and moreover the positive reaction with the peripheral nervous tissue and thesensitivity was 10%. All tested commercially available antibodies in our laboratory showed unsuitable sensitivity for the purpose of the semiquantitative identification of CNS tissue."@en . "Comparison of commercially available antibodies for the detection of central nervous system tissue in meat products by enzyme-linked immunosorbent assay" . . "1"^^ . . . "antibody; central nervous tissue; ELISA"@en . "Comparison of commercially available antibodies for the detection of central nervous system tissue in meat products by enzyme-linked immunosorbent assay"@en . "3"^^ . "1"^^ . . "Journal of Food Protection" . "P(QC1111), Z(MZE0002716201)" . . "630;632" . "\u010Cty\u0159i typy komer\u010Dn\u011B dostupn\u00FDch protil\u00E1tek potenci\u00E1ln\u011B vhodn\u00FDch pro identifikaci centr\u00E1ln\u00ED nervov\u00E9 tk\u00E1n\u011B byly testov\u00E1ny pomoc\u00ED ELISA metody. Protil\u00E1tky byly testov\u00E1ny z hlediska specifity a sensitivity. Citlivost protil\u00E1tky proti kysel\u00E9mu gliov\u00E9mufibril\u00E1rn\u00EDmu proteinu (ICN) byla 25 %. Protil\u00E1tka nereagovala se svalovinou, br\u00E1nic\u00ED ani s perifern\u00EDmi nervy. Citlivost monoklon\u00E1ln\u00ED protil\u00E1tky 200 proti neurofilament\u016Fm byla rovn\u011B\u017E 25%. Citlivost dal\u0161\u00ED testovan\u00E9 polyklon\u00E1ln\u00ED protil\u00E1tky protineurofilament\u016Fm (Sigma ) byla 2% a reakce se vzorky svaloviny, br\u00E1nice a perifern\u00EDch nerv\u016F byla shodn\u00E1 jako u p\u0159edchoz\u00EDch test\u016F. Posledn\u00ED testovan\u00E1 protil\u00E1tka proti myelinov\u00E9mu bazick\u00E9mu proteinu (Sigma) m\u011Bla citlivost 10% a oproti p\u0159edchoz\u00EDm testovan\u00FDmprotil\u00E1tk\u00E1m reagovala pozitivn\u011B s perifern\u00ED nervovou tk\u00E1n\u00ED. Citlivost v\u0161ech t\u011Bchto testovan\u00FDch protil\u00E1tek byla shled\u00E1na jako nedosta\u010Duj\u00EDc\u00ED pro semikvantitativn\u00ED detekci centr\u00E1ln\u00ED nervov\u00E9 tk\u00E1n\u011B."@cs . . "Comparison of commercially available antibodies for the detection of central nervous system tissue in meat products by enzyme-linked immunosorbent assay" . "[A575F7C1A87C]" . . . "RIV/00027162:_____/05:06000015" . .