"Shoot organogenesis induction from genetically verified individuals of endangered bog pine (Pinus uncinata subsp. uliginosa)"@en . . "1212-4834" . . "Shoot organogenesis induction from genetically verified individuals of endangered bog pine (Pinus uncinata subsp. uliginosa)"@en . "Shoot organogenesis induction from genetically verified individuals of endangered bog pine (Pinus uncinata subsp. uliginosa)" . . "Journal of Forest Science" . "287201" . . "2"^^ . . "RIV/00027073:_____/10:#0000838!RIV11-MZP-00027073" . . . "[31CB0E2A7FCF]" . "Shoot organogenesis induction from genetically verified individuals of endangered bog pine (Pinus uncinata subsp. uliginosa)" . . "8" . "2"^^ . "RIV/00027073:_____/10:#0000838" . "56" . . . "Vejsadov\u00E1, Hana" . . "Luk\u00E1\u0161ov\u00E1, Miroslava" . "7"^^ . "The objective of the work was to apply selected genetic markers to verify the genetic purity of bog pine (Pinus uncinata subsp. uliginosa) individuals from the Borkovick\u00E1 blata population in order to initiate in vitro cultures from them. The plastid DNA analysis was used as a molecular-genetic tool. The trnL-trnF region of chloroplast DNA was tested by PCR-RFLP method; furthermore, the chloroplast microsatellite region Pt41093 was also analysed. All the tested individuals from the studied population matched the Pinus uncinata subsp. uliginosa haplotype. In bog pine, regeneration and development of axillary shoots were significantly enhanced in the presence of 5 g/L activated charcoal." . . . . . "DNA extraction; in vitro regeneration; plastid DNA; seedling shoot apices"@en . . "CZ - \u010Cesk\u00E1 republika" . "P(SP/2D4/83/07)" . "The objective of the work was to apply selected genetic markers to verify the genetic purity of bog pine (Pinus uncinata subsp. uliginosa) individuals from the Borkovick\u00E1 blata population in order to initiate in vitro cultures from them. The plastid DNA analysis was used as a molecular-genetic tool. The trnL-trnF region of chloroplast DNA was tested by PCR-RFLP method; furthermore, the chloroplast microsatellite region Pt41093 was also analysed. All the tested individuals from the studied population matched the Pinus uncinata subsp. uliginosa haplotype. In bog pine, regeneration and development of axillary shoots were significantly enhanced in the presence of 5 g/L activated charcoal."@en . .