. "Cyclodextrins (CDs) are synthesized by the bacterial extracellular enzym cyclodextrin glycosyltransferase (CGTase, E.C. 2.4.1.19) from starch or starch derivatives via an inter- and intramolecural transglycosylation reaction. The production of ?- CD, \u00DF- CD and ?- CD by CGTase from Paenibacillus macerans was studied, in regard to the effect of the source starch (amaranth, corn) on the yield of CDs. The CGTase was produced by a 3-day sterile cultivation in the laboratory Bench-top fermentor BiostatB in a complex medium containing starch and partially purified by ammonium sulphate precipitation at 60% saturation. Apparent molecular weight as determined by SDS\u2013PAGE was in the range from 105 to 114 kDa. All the commercially important ?-, \u00DF-, and ?-CDs were detected chromatographically after hydrolysis of the corn and amaranth (Amaranthus cruentus) starch with the isolated enzyme."@en . . "Mol\u00EDk, Petr" . "331971" . . "Drahor\u00E1d, Josef" . "Cyclodextrins (CDs) are synthesized by the bacterial extracellular enzym cyclodextrin glycosyltransferase (CGTase, E.C. 2.4.1.19) from starch or starch derivatives via an inter- and intramolecural transglycosylation reaction. The production of ?- CD, \u00DF- CD and ?- CD by CGTase from Paenibacillus macerans was studied, in regard to the effect of the source starch (amaranth, corn) on the yield of CDs. The CGTase was produced by a 3-day sterile cultivation in the laboratory Bench-top fermentor BiostatB in a complex medium containing starch and partially purified by ammonium sulphate precipitation at 60% saturation. Apparent molecular weight as determined by SDS\u2013PAGE was in the range from 105 to 114 kDa. All the commercially important ?-, \u00DF-, and ?-CDs were detected chromatographically after hydrolysis of the corn and amaranth (Amaranthus cruentus) starch with the isolated enzyme." . . "[6F0BFD26BCA4]" . . "Urban, Marian" . "Cyclodextrin; fermentation; starch"@en . . "RIV/00027022:_____/09:00000129!RIV10-MSM-00027022" . "Matu\u0161ov\u00E1, Krystina" . . "Ad\u00E1mek, Lubom\u00EDr" . . . . "Beran, Milo\u0161" . . . . "6"^^ . . "P(2B06173)" . . . "RIV/00027022:_____/09:00000129" . . "Optimalization of production of cyclodextrin glycosyl transferase by Paenibacillus macerans strain and cyclodextrin formation (Abstract: Proceedings of The 5th Asian Cyclodextrin Conference (ACC2009). May 13-16, 2009. Busan, Korea)"@en . "Optimalization of production of cyclodextrin glycosyl transferase by Paenibacillus macerans strain and cyclodextrin formation (Abstract: Proceedings of The 5th Asian Cyclodextrin Conference (ACC2009). May 13-16, 2009. Busan, Korea)"@en . "5"^^ . . . "Optimalization of production of cyclodextrin glycosyl transferase by Paenibacillus macerans strain and cyclodextrin formation (Abstract: Proceedings of The 5th Asian Cyclodextrin Conference (ACC2009). May 13-16, 2009. Busan, Korea)" . "Optimalization of production of cyclodextrin glycosyl transferase by Paenibacillus macerans strain and cyclodextrin formation (Abstract: Proceedings of The 5th Asian Cyclodextrin Conference (ACC2009). May 13-16, 2009. Busan, Korea)" .