"Erban, Tom\u00E1\u0161" . "1226-8615" . "28648" . . "Glutathione S-transferases (GSTs) were purified using GSTrap 4B column, and the protein profile of the supernatant before purification was compared with the unbound fraction post-purification via two-dimensional gel electrophoresis (2D-E). The identity of these localized protein spots was confirmed by MS analysis and further analyzed by NanoLC-ESI-QUAD-TOF-MS/MS protein identification. Results indicate that 2 out of 5 protein spots were GSTs with region for class Delta and Epsilon subfamilies similar to other insects; however, the remaining 3 spots did not show any match in the current NCBInr. Both Delta and Epsilon class GSTs are specifically involved in insecticide resistance and their relatively high abundance in the 2D-E map suggests that these enzymes could play a role in the resistance of Meligethes aeneus to the most commonly used pyrethroids. The approach applied in this study for the specific localization of GSTs in 2D-E can be used for similar analyses in other organisms." . . "10.1016/j.aspen.2014.02.010" . . . "RIV/00027006:_____/14:00002857" . . "2"^^ . "Methodology for glutathione S-transferase purification and localization in two-dimensional gel electrophoresis performed on the pollen beetle, Meligethes aeneus (Coleoptera: Nitidulidae)" . "RIV/00027006:_____/14:00002857!RIV15-MZE-00027006" . . "P(QH91152)" . "Star\u00E1, Jitka" . "[F7AFE87560B7]" . "2"^^ . "Methodology for glutathione S-transferase purification and localization in two-dimensional gel electrophoresis performed on the pollen beetle, Meligethes aeneus (Coleoptera: Nitidulidae)"@en . . . . "JOURNAL OF ASIA-PACIFIC ENTOMOLOGY" . . . "Glutathione S-transferase; Affinity chromatography; 2D-E; Meligethes aeneus; Proteomics; Delta and Epsilon GST subfamilies"@en . . "Glutathione S-transferases (GSTs) were purified using GSTrap 4B column, and the protein profile of the supernatant before purification was compared with the unbound fraction post-purification via two-dimensional gel electrophoresis (2D-E). The identity of these localized protein spots was confirmed by MS analysis and further analyzed by NanoLC-ESI-QUAD-TOF-MS/MS protein identification. Results indicate that 2 out of 5 protein spots were GSTs with region for class Delta and Epsilon subfamilies similar to other insects; however, the remaining 3 spots did not show any match in the current NCBInr. Both Delta and Epsilon class GSTs are specifically involved in insecticide resistance and their relatively high abundance in the 2D-E map suggests that these enzymes could play a role in the resistance of Meligethes aeneus to the most commonly used pyrethroids. The approach applied in this study for the specific localization of GSTs in 2D-E can be used for similar analyses in other organisms."@en . . . . "3" . "Methodology for glutathione S-transferase purification and localization in two-dimensional gel electrophoresis performed on the pollen beetle, Meligethes aeneus (Coleoptera: Nitidulidae)" . "KR - Korejsk\u00E1 republika" . . "17" . . "5"^^ . . "000340737700026" . . "Methodology for glutathione S-transferase purification and localization in two-dimensional gel electrophoresis performed on the pollen beetle, Meligethes aeneus (Coleoptera: Nitidulidae)"@en .