. "3" . . . "1392-3196" . . "000259827600038" . "392251" . . "Mr\u00E1z, I." . "Reliability of diagnostic techniques for Erwinia amylovora and Clavibacter michiganensis subsp. sepedonicus"@en . "1"^^ . . "Zemdirbyste-Agriculture" . . "2"^^ . . . . "Spolehlivost diagnostick\u00FDch technik pro Erwinia amylovora a Clavibacter michiganensis subsp. sepedonicus"@cs . "Koko\u0161kov\u00E1, Blanka" . "7"^^ . "RIV/00027006:_____/08:00000268!RIV09-MZE-00027006" . . "95" . . "Erwinia amylovora (Ea) causing fire blight and Clavibacter michiganensis subsp. sepedoniciis (Cms) causing ring rot of potato belong to the quarantine organisms. Detection and determination of both pathogens using different methods were compared. Commercial MAb were used for Cms and PAb for Ea. All Ea strains were reliably identified by PCR (Bereswill and own primers) after optimisation of PCR protocol and using BIOLOG System. PTA-ELISA and IIF were less reliable because of cross-reacting bacteria associated with Ea in host plants. AH Cms strains were reliably identified by IIF and PCR (Mills and Pastrik primers) regardless of morphological variability. The fluidal Cms strains were more reliably identified using DAS-ELISA and BIOLOG System than less fluidal strains."@en . . "Z(MZE0002700603)" . "Erwinia amylovora (Ea), p\u016Fvodce sp\u00E1ly r\u016F\u017Eovit\u00FDch rostlin a Clavibacter michiganensis subsp. sepedoniciis (Cms), p\u016Fvodce krou\u017Ekovitosti bramboru, pat\u0159\u00ED ke karant\u00E9nn\u00EDm organism\u016Fm. Porovn\u00E1vala se detekce a determinace obou patogen\u016F pomoc\u00ED r\u016Fzn\u00FDch diagnostick\u00FDch metod. Komer\u010Dn\u00ED MAb byly pou\u017Eity pro Cms a PAb pro Ea. V\u0161echny Ea kmeny byly spolehliv\u011B identifikov\u00E1ny pomoc\u00ED PCR po optimalizaci PCR protokolu a syst\u00E9mem BIOLOG. PTA-ELISA a IIF byly m\u00E9n\u011B spolejliv\u00E9 kv\u016Fli k\u0159\u00ED\u017Eov\u00FDch reakc\u00EDm bakteri\u00ED doprov\u00E1zej\u00EDc\u00EDch Ea v hostitelsk\u00FDch rostlin\u00E1ch. V\u0161echny Cms kmeny byly spolehliv\u011B identifikov\u00E1ny pomoc\u00ED IIF a PCR bez ohledu na morfologickou variabilitu. Fluidn\u00ED kmeny Cms byly spolehliv\u011Bji identifikov\u00E1ny pomoc\u00ED DAS-ELISA a syst\u00E9mu BIOLOG ne\u017E nefluidn\u00ED."@cs . "Reliability of diagnostic techniques for Erwinia amylovora and Clavibacter michiganensis subsp. sepedonicus" . "Erwinia amylovora; Clavibacter michiganensis subsp. sepedonicus; detection; determination; PCR; BIOLOG; ELISA; IF"@en . . "Erwinia amylovora (Ea) causing fire blight and Clavibacter michiganensis subsp. sepedoniciis (Cms) causing ring rot of potato belong to the quarantine organisms. Detection and determination of both pathogens using different methods were compared. Commercial MAb were used for Cms and PAb for Ea. All Ea strains were reliably identified by PCR (Bereswill and own primers) after optimisation of PCR protocol and using BIOLOG System. PTA-ELISA and IIF were less reliable because of cross-reacting bacteria associated with Ea in host plants. AH Cms strains were reliably identified by IIF and PCR (Mills and Pastrik primers) regardless of morphological variability. The fluidal Cms strains were more reliably identified using DAS-ELISA and BIOLOG System than less fluidal strains." . . "Reliability of diagnostic techniques for Erwinia amylovora and Clavibacter michiganensis subsp. sepedonicus" . . . "[4C6DFE894AEB]" . "Spolehlivost diagnostick\u00FDch technik pro Erwinia amylovora a Clavibacter michiganensis subsp. sepedonicus"@cs . . . "Reliability of diagnostic techniques for Erwinia amylovora and Clavibacter michiganensis subsp. sepedonicus"@en . . "RIV/00027006:_____/08:00000268" . "LT - Litevsk\u00E1 republika" .