"RIV/00027006:_____/07:2420" . "[D558CEE4C435]" . "Comparison of Detection Methods for Acarus siro (Acari:Acaridida: Acarididae) Contamination in Grain" . "6" . . "Comparison of Detection Methods for Acarus siro (Acari:Acaridida: Acarididae) Contamination in Grain"@en . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "100" . . "Comparison of Detection Methods for Acarus siro (Acari:Acaridida: Acarididae) Contamination in Grain"@en . . . . "P(QF4071)" . . "Journal of Economic Entomology" . . "Pr\u00E1ce se zab\u00FDv\u00E1 porovn\u00E1n\u00EDm detek\u010Dn\u00EDch metod pro identifikaci kontaminace rozto\u010Dem Acarus siro. Jsou testov\u00E1ny klasick\u00E9 detek\u010Dn\u00ED metody (extrakce na Berlese-Tullgren extraktoru, pros\u00E9v\u00E1n\u00ED a flotace). Dal\u0161\u00ED pou\u017Eitou metodou byla ELISA s nov\u011B p\u0159ipravenou polyklon\u00E1ln\u00ED protil\u00E1tkou pro detekci A. siro. Bylo zji\u0161t\u011Bno, \u017Ee dosp\u011Blce a juveniln\u00ED st\u00E1dia bylo mo\u017En\u00E9 detekovat v\u0161emi uveden\u00FDmi metodami s rozd\u00EDln\u00FDm detek\u010Dn\u00EDm limitem v rozsahu 221 a\u017E 1157 rozto\u010D\u016F/kg obil\u00ED. Vaj\u00ED\u010Dka bylo mo\u017En\u00E9 detekovat pouze pomoc\u00ED flotace (detek\u010Dn\u00ED limit 1950 vaj\u00ED\u010Dek/kg obil\u00ED). Exkrementy rozto\u010De byly detekovateln\u00E9 pouze pomoc\u00ED imunochemick\u00E9ho testu s detek\u010Dn\u00EDm limitem 1.04 \u00B5g exkrement\u016F/g obil\u00ED, co\u017E znamen\u00E1, \u017Ee pou\u017Eit\u00E1 technika umo\u017En\u00ED detekci jednoho metabolicky aktivn\u00EDho rozto\u010De/g obil\u00ED."@cs . "Acarus siro L. (Acari: Acaridida: Acarididae) is an important pest of stored grain because it contaminates the grain by allergens and transfers pathogenous microorganisms. Rapid detection of contamination enables to intercept an early grain infestation by the pest. In this study, we compared the usability and efciency of various detection approaches. Under laboratory conditions, grain samples of various sizes were infested by different levels of the following contaminants: eggs, adults, and feces of A. siro. The samples were analyzed by enzyme-linked immunosorbent essay (ELISA) by using anti-A. siro polyclonal antibody (immunochemical method), extracted in Berlese-Tullgren funnels, sieved, and processed by the flotation (conventional methods). The adults or juveniles of A. siro could be detected by all the three tested conventional methods and ELISA with detection limits in the range from 221 to 1,157 mites/kg grain. Eggs were detected by the flotation only; the detection limit was 1,950 eggs/kg gra"@en . . . "K\u0159\u00ED\u017Ekov\u00E1 Kudl\u00EDkov\u00E1, Iva" . "Stejskal, V\u00E1clav" . "Acarus siro L. (Acari: Acaridida: Acarididae) is an important pest of stored grain because it contaminates the grain by allergens and transfers pathogenous microorganisms. Rapid detection of contamination enables to intercept an early grain infestation by the pest. In this study, we compared the usability and efciency of various detection approaches. Under laboratory conditions, grain samples of various sizes were infested by different levels of the following contaminants: eggs, adults, and feces of A. siro. The samples were analyzed by enzyme-linked immunosorbent essay (ELISA) by using anti-A. siro polyclonal antibody (immunochemical method), extracted in Berlese-Tullgren funnels, sieved, and processed by the flotation (conventional methods). The adults or juveniles of A. siro could be detected by all the three tested conventional methods and ELISA with detection limits in the range from 221 to 1,157 mites/kg grain. Eggs were detected by the flotation only; the detection limit was 1,950 eggs/kg gra" . . . "Porovn\u00E1n\u00ED detek\u010Dn\u00EDch metod pro rozto\u010De Acarus siro (Acari: Acaridida: Acarididae) v obil\u00ED"@cs . "Comparison of Detection Methods for Acarus siro (Acari:Acaridida: Acarididae) Contamination in Grain" . . . "extraction; ELISA; grain; mite; storage"@en . "3"^^ . "414310" . "RIV/00027006:_____/07:2420!RIV08-MZE-00027006" . . . "3"^^ . . "Porovn\u00E1n\u00ED detek\u010Dn\u00EDch metod pro rozto\u010De Acarus siro (Acari: Acaridida: Acarididae) v obil\u00ED"@cs . "Hubert, Jan" . . . "1928;1937" . "10"^^ . "0022-0493" . .