. . "Proliferation and cold hardening of in vitro grown apple shoot tips" . . . . "Proliferation and cold hardening of in vitro grown apple shoot tips"@en . "Proliferace a mrazov\u00E9 otu\u017Eov\u00E1n\u00ED in vitro kultur jablon\u00ED"@cs . . "Acta Horticulturae" . "0567-7572" . "Proliferation and cold hardening of in vitro grown apple shoot tips"@en . . "NL - Nizozemsko" . "Bilav\u010D\u00EDk, Alois" . "2"^^ . "4"^^ . "[EEEB6334C38F]" . "0" . . "micropropagation, germplasm, encapsulation, cryopreservation, freezing"@en . "Shoot tips of apple cultivar 'Rubin' were successfully established in vitro. Five proliferation MS (Murashige and Skoog) media containing different concentrations of BAP (0.25, 0.5, 1, 2 mg L-1) or a combination of 1 mg L-1 BAP and 0.1 mg L-1 IBA were tested. The highest proliferation rate was obtained on MS medium with 1 mg L-1 BAP, which produced 4.2 0.3 shoots/explant after one month of cultivation. In vitro plants of cv. 'Rubin' were cultivated for 4 weeks at low temperature (4\u00B0C) and short photoperiod (8/16-h \u2013 light/dark). The assessment of frost resistance of cold hardened and non-hardened in vitro apple plants was done by freezing tests. In vitro plants were exposed to the controlled cooling rate 4\u00B0C/h. The lethal temperature (LT) was -13.0\u00B0C for cold hardened and 6.5\u00B0C for non-hardened in vitro plants. Cold hardened in vitro cultures were used for subsequent cryopreservation. Cryopreservation was made by encapsulation-dehydration vitrification method. The survival and regeneration of c"@cs . . "495489" . "RIV/00027006:_____/06:1736" . . "Sedl\u00E1k, Ji\u0159\u00ED" . "Proliferace a mrazov\u00E9 otu\u017Eov\u00E1n\u00ED in vitro kultur jablon\u00ED"@cs . . . . "Shoot tips of apple cultivar 'Rubin' were successfully established in vitro. Five proliferation MS (Murashige and Skoog) media containing different concentrations of BAP (0.25, 0.5, 1, 2 mg L-1) or a combination of 1 mg L-1 BAP and 0.1 mg L-1 IBA were tested. The highest proliferation rate was obtained on MS medium with 1 mg L-1 BAP, which produced 4.2 0.3 shoots/explant after one month of cultivation. In vitro plants of cv. 'Rubin' were cultivated for 4 weeks at low temperature (4\u00B0C) and short photoperiod (8/16-h \u2013 light/dark). The assessment of frost resistance of cold hardened and non-hardened in vitro apple plants was done by freezing tests. In vitro plants were exposed to the controlled cooling rate 4\u00B0C/h. The lethal temperature (LT) was -13.0\u00B0C for cold hardened and 6.5\u00B0C for non-hardened in vitro plants. Cold hardened in vitro cultures were used for subsequent cryopreservation. Cryopreservation was made by encapsulation-dehydration vitrification method. The survival and regeneration of c" . "Shoot tips of apple cultivar 'Rubin' were successfully established in vitro. Five proliferation MS (Murashige and Skoog) media containing different concentrations of BAP (0.25, 0.5, 1, 2 mg L-1) or a combination of 1 mg L-1 BAP and 0.1 mg L-1 IBA were tested. The highest proliferation rate was obtained on MS medium with 1 mg L-1 BAP, which produced 4.2 0.3 shoots/explant after one month of cultivation. In vitro plants of cv. 'Rubin' were cultivated for 4 weeks at low temperature (4\u00B0C) and short photoperiod (8/16-h \u2013 light/dark). The assessment of frost resistance of cold hardened and non-hardened in vitro apple plants was done by freezing tests. In vitro plants were exposed to the controlled cooling rate 4\u00B0C/h. The lethal temperature (LT) was -13.0\u00B0C for cold hardened and 6.5\u00B0C for non-hardened in vitro plants. Cold hardened in vitro cultures were used for subsequent cryopreservation. Cryopreservation was made by encapsulation-dehydration vitrification method. The survival and regeneration of c"@en . "4"^^ . . . "P(QC1052)" . "RIV/00027006:_____/06:1736!RIV07-MZE-00027006" . . . "0" . "Proliferation and cold hardening of in vitro grown apple shoot tips" . "Z\u00E1me\u010Dn\u00EDk, Ji\u0159\u00ED" . . "Papr\u0161tein, Franti\u0161ek" .