"2"^^ . . "V\u00A0posledn\u00EDch letech byla vyvinuta tzv.chipov\u00E1 elektrofor\u00E9za, kter\u00E1 je miniaturizovanou modifikac\u00ED kapil\u00E1rn\u00ED elektrofor\u00E9zy. Chipov\u00E1 elektrofor\u00E9za, p\u0159edstavuje kombinaci mikrofluidn\u00ED separa\u010Dn\u00ED techniky a citliv\u00E9 fluorescen\u010Dn\u00ED detekce pro kvantifikaci obsahov\u00E9 l\u00E1tky (DNA, RNA nebo protein\u016F). Dosud je pou\u017Eiteln\u00E1 pouze pro elektroforetick\u00E9 separace v\u00A0p\u0159\u00EDtomnosti dodecylsulf\u00E1tu sodn\u00E9ho (SDS). V\u00A0na\u0161\u00ED laborato\u0159i ve V\u00DARV byla \u00FAsp\u011B\u0161n\u011B vyzkou\u0161ena pro anal\u00FDzy frakce n\u00EDzkomolekul\u00E1rn\u00EDch (LMW-GS) a vysokomolekul\u00E1rn\u00EDch (HMW-GS) podjednotek glutenin\u016F p\u0161enice a hordein\u016F je\u010Dmene \u2013 oba postupy klasick\u00E9 elektrofor\u00E9zy(SDS-PAGE) se pou\u017E\u00EDvaj\u00ED pro identifikaci genotyp\u016F (odr\u016Fd, lini\u00ED) i pro markerov\u00E1n\u00ED n\u011Bkter\u00FDch znak\u016F jakosti, kter\u00E9 jsou s\u00A0t\u011Bmito proteinov\u00FDmi slo\u017Ekami ve vazb\u011B. Metoda chipov\u00E9 elektrofor\u00E9zy byla porovn\u00E1na s klasickou metodou elektrofor\u00E9zy (SDS-PAGE). Pomoc\u00ED obou technik byly z\u00EDsk\u00E1ny srovnateln\u00E9 v\u00FDsledky. V\u00FDsledky \u010Dipov\u00E9 elektrofor\u00E9zy se vyzna\u010Dovaly vysokou reprodukovatelnost\u00ED."@cs . . "Mat\u011Bjov\u00E1, Eva" . "For identification of varieties of cereals we used new electrophoretic system. The Experion automated electrophoresis system applies a combination of microfluidic separation technology and sensitive fluorescent sample detection to perform rapid and automated analysis of protein. It automatically performs all steps of gel-based electrophoresis (sample separation, staining, destaining, imaging, band detection, and even some data analysis) to deliver reproducible separation and quantitation results in just 30 minutes. In our laboratory we used this system for analysis of protein of wheat (LMW and HMW glutenin subunits) and barley (hordeins). This approach was compared with the classic electrophoresis these proteins in polyacrylamide gel and sodium dodecyl sulphate. Results were characterized by considerable reproducibility and especially protein patterns of wheat obtained these techniques were very comparable."@en . . . "Book of proceedings: XX. Biochemick\u00FD zjazd" . . "For identification of varieties of cereals we used new electrophoretic system. The Experion automated electrophoresis system applies a combination of microfluidic separation technology and sensitive fluorescent sample detection to perform rapid and automated analysis of protein. It automatically performs all steps of gel-based electrophoresis (sample separation, staining, destaining, imaging, band detection, and even some data analysis) to deliver reproducible separation and quantitation results in just 30 minutes. In our laboratory we used this system for analysis of protein of wheat (LMW and HMW glutenin subunits) and barley (hordeins). This approach was compared with the classic electrophoresis these proteins in polyacrylamide gel and sodium dodecyl sulphate. Results were characterized by considerable reproducibility and especially protein patterns of wheat obtained these techniques were very comparable." . . . . . . "\u00DAstav molekul\u00E1rnej fyziol\u00F3gie a genetiky SAV" . "Bradov\u00E1, Jana" . "Utilization of Chip Electrophoresis for Characterization of Cereal varieties" . "Pie\u0161\u0165any, Slovensk\u00E1 republika" . "Pie\u0161\u0165any, Slovensk\u00E1 republika" . . "80-969532-6-5" . "Utilization of Chip Electrophoresis for Characterization of Cereal varieties"@en . "Vyu\u017Eit\u00ED \u010Dipov\u00E9 elektrofor\u00E9zy k charakterizaci odr\u016Fd obilovin"@cs . "505474" . "[728432B5A8F8]" . . "1"^^ . "Z(MZE0002700602)" . . . . "Vyu\u017Eit\u00ED \u010Dipov\u00E9 elektrofor\u00E9zy k charakterizaci odr\u016Fd obilovin"@cs . "Utilization of Chip Electrophoresis for Characterization of Cereal varieties"@en . "RIV/00027006:_____/06:1296!RIV08-MZE-00027006" . "wheat; barley; electrophoresis; variety purity"@en . "Utilization of Chip Electrophoresis for Characterization of Cereal varieties" . . "2"^^ . "2006-09-12+02:00"^^ . . . "410;410" . "RIV/00027006:_____/06:1296" .