"34;37" . "First report of Pepper mild mottle virus in pepper seeds produced in the Czech Republic" . "4"^^ . "Z(MZE0002700603)" . . . "RIV/00027006:_____/06:1275!RIV08-MZE-00027006" . "First report of Pepper mild mottle virus in pepper seeds produced in the Czech Republic"@en . . "4"^^ . . "[022D930500B1]" . "2"^^ . . . . "1" . . "Prvn\u00ED n\u00E1lez Pepper mild mottle viru v semenech paprik produkovan\u00FDch v \u010Cesk\u00E9 Republice"@cs . "1212-2580" . . . "\u010Cerven\u00E1, G." . "CZ - \u010Cesk\u00E1 republika" . "Prvn\u00ED n\u00E1lez Pepper mild mottle viru v semenech paprik produkovan\u00FDch v \u010Cesk\u00E9 Republice"@cs . "Symptoms of viral infection were observed on plants of pepper, cv. OL 228, raised from commercial seeds of Czech origin in a greenhouse in the year 2002. Infected plants showed mosaic or mottling on leaves, and necrotic depressions on fruits. Straight, rod-shaped viral particles of about 300 nm, indicating a tobamovirus infection, were found by electron microscope. ELISA produced negative reactions for Tobacco mosaic virus (TMV) but positive reactions with an antiserum to Pepper mild mottle virus (PMMoV). In biological characterisation using pepper cultivars with the L1, L2, L3 and L4 tobamovirus resistance genes it was found that the Czech isolate of PMMoV belongs to pathotype Pl.2. This is the first report of PMMoV in the Czech Republic. Its distribution, however, may still be limited as a survey did not reveal other infections in the main pepper producing areas. As PMMoV spreads with infected seeds, the possibility of its chemical deactivation by NaOH was tested and confirmed." . . "RIV/00027006:_____/06:1275" . . "First report of Pepper mild mottle virus in pepper seeds produced in the Czech Republic"@en . "42" . "Plant Protection Science" . . "Radov\u00E1, J." . "Symptoms of viral infection were observed on plants of pepper, cv. OL 228, raised from commercial seeds of Czech origin in a greenhouse in the year 2002. Infected plants showed mosaic or mottling on leaves, and necrotic depressions on fruits. Straight, rod-shaped viral particles of about 300 nm, indicating a tobamovirus infection, were found by electron microscope. ELISA produced negative reactions for Tobacco mosaic virus (TMV) but positive reactions with an antiserum to Pepper mild mottle virus (PMMoV). In biological characterisation using pepper cultivars with the L1, L2, L3 and L4 tobamovirus resistance genes it was found that the Czech isolate of PMMoV belongs to pathotype Pl.2. This is the first report of PMMoV in the Czech Republic. Its distribution, however, may still be limited as a survey did not reveal other infections in the main pepper producing areas. As PMMoV spreads with infected seeds, the possibility of its chemical deactivation by NaOH was tested and confirmed."@en . . "First report of Pepper mild mottle virus in pepper seeds produced in the Czech Republic" . . . . . "Pepper mild mottle virus; characterisation; pathotype Pl.2; Capsicum annuum; ELISA; electron microscopy; seed transmission; virus deactivation; sodium hydroxide"@en . . "475935" . . "Joke\u0161, Milan" . . "Svoboda, Ji\u0159\u00ED" . . "V roce 2002 byly pozorov\u00E1ny p\u0159\u00EDznaky virov\u00E9 infekce na rostlin\u00E1ch paprik 'OL228' vze\u0161l\u00FDch z komer\u010Dn\u011B prod\u00E1van\u00E9ho osiva. Infikovan\u00E9 rostliny m\u011Bly mozaiku na listech a nekrotick\u00E9 jizvy na plodech. Elektronov\u00FDm mikroskopem byly objeveny ty\u010Dinkov\u00E9 virov\u00E9 \u010D\u00E1stice d\u00E9lky 300 nm typick\u00E9 pro tobamoviry. Pomoc\u00ED ELISA byl identifikov\u00E1n Pepper mild mottle virus (PMMoV). Biologickou charakterizac\u00ED na r\u016Fzn\u00FDch odr\u016Fd\u00E1ch paprik byl ur\u010Den patotyp P1.2. Toto je prvn\u00ED zpr\u00E1va o n\u00E1lezu PMMoV v \u010Cesk\u00E9 republice. Jeho roz\u0161\u00ED\u0159en\u00ED je omezen\u00E9, v hlavn\u00EDch produk\u010Dn\u00EDch oblastech p\u011Bstov\u00E1n\u00ED paprik nebyl virus zji\u0161t\u011Bn. Byla prov\u011B\u0159ena mo\u017Enost dezinfekce infikovan\u00E9ho osiva pomoc\u00ED NaOH."@cs .