. "CZ - \u010Cesk\u00E1 republika" . . "473413" . "microspore culture; doubled haploids; winter rape; Brassica napus L.; hybrid breeding"@en . "V\u011Btrovcov\u00E1, M." . "Efficiency of Microspore Culture for Doubled Haploid Production in the Breeding Project \u201CCzech Winter Rape\u201D"@en . . "Kl\u00EDma, Miroslav" . "Czech Journal of Genetics and Plant Breeding" . . . . . "42" . "14"^^ . "2" . "RIV/00027006:_____/06:1247" . "RIV/00027006:_____/06:1247!RIV08-MZE-00027006" . . "Mach\u00E1\u010Dkov\u00E1, I." . . "1212-1975" . "Efficiency of Microspore Culture for Doubled Haploid Production in the Breeding Project \u201CCzech Winter Rape\u201D" . "Efficiency of Microspore Culture for Doubled Haploid Production in the Breeding Project \u201CCzech Winter Rape\u201D"@en . . "58;71" . "5"^^ . "Efektivita mikrosporov\u00FDch kultur pro produkci dihaploid\u016F v r\u00E1mci sdru\u017Een\u00ED %22\u010Cesk\u00E1 \u0159epka%22"@cs . "Efficiency of Microspore Culture for Doubled Haploid Production in the Breeding Project \u201CCzech Winter Rape\u201D" . . "P(1G46061), P(QE0052)" . . . "Griga, M." . "A selected set of twenty inter-line F1 hybrids of Brassica napus L. was evaluated for their capacity to produce microspore-derived doubled haploid (DH) plants subsequently utilized in the breeding project \u201CCzech Winter Rape\u201D. All tested genotypes were able to produce microspore-derived embryos based on the standardized protocol previously adopted for the Czech winter rape varieties/breeding lines; nevertheless, significant differences were found in the frequency of microspore-derived embryo production as related to particular genotypes. However, the fertile DH regenerants were obtained only in 12 hybrid lines of this set. The other 8 lines failed to produce fertile DH plants for several reasons, mainly due to altered morphology of microspore embryos connected with limited germination/conversion potential and due to unsuccessful colchicine treatment resulting in haploid or mixoploid (chimeric) regenerants exhibiting partial or full sterility. In general, colchicine treatment induced diploid (doubled h"@en . "[5039A098E726]" . "Sm\u00FDkalov\u00E1, I." . . "1"^^ . . "A selected set of twenty inter-line F1 hybrids of Brassica napus L. was evaluated for their capacity to produce microspore-derived doubled haploid (DH) plants subsequently utilized in the breeding project \u201CCzech Winter Rape\u201D. All tested genotypes were able to produce microspore-derived embryos based on the standardized protocol previously adopted for the Czech winter rape varieties/breeding lines; nevertheless, significant differences were found in the frequency of microspore-derived embryo production as related to particular genotypes. However, the fertile DH regenerants were obtained only in 12 hybrid lines of this set. The other 8 lines failed to produce fertile DH plants for several reasons, mainly due to altered morphology of microspore embryos connected with limited germination/conversion potential and due to unsuccessful colchicine treatment resulting in haploid or mixoploid (chimeric) regenerants exhibiting partial or full sterility. In general, colchicine treatment induced diploid (doubled h" . "Zvolen\u00E1 skupina 20 F1 hybrid\u016F \u0159epky ozim\u00E9 byla prov\u011B\u0159ena z hlediska jejich schopnosti produkovat dihaploidn\u00ED (DH) rostliny v mikrosporov\u00FDch kultur\u00E1ch, realizovan\u00FDch v r\u00E1mci sdru\u017Een\u00ED %22\u010Cesk\u00E1 \u0159epka%22. V\u0161echny testovan\u00E9 genotypy m\u011Bly schopnost produkce mikrosporov\u00FDch embry\u00ED pomoc\u00ED standardn\u00EDho protokolu, optimalizovan\u00E9ho pro kultivary \u010Desk\u00FDch lini\u00ED a variet; i tak byly zji\u0161t\u011Bny v\u00FDznamn\u00E9 rozd\u00EDly v embryogenn\u00ED responsibilit\u011B mezi testovan\u00FDmi genotypy. Fertiln\u00ED DH regeneranty byly z\u00EDsk\u00E1ny pouze z 12 testovan\u00FDch hybrid\u016F. U ostatn\u00EDch 8 lini\u00ED byla tvorba fertiln\u00EDch DH lini\u00ED blokov\u00E1na z nejr\u016Fzn\u011Bj\u0161\u00EDch d\u016Fvod\u016F, zejm\u00E9na v d\u016Fsledku nevhodn\u00E9 morfologie z\u00EDskan\u00FDch embry\u00EDch a jej\u00ED n\u00E1vaznosti na p\u0159\u00EDmou regeneraci a v d\u016Fsledku nedostate\u010Dn\u00E9 diploidiza\u010Dn\u00ED \u00FA\u010Dinnosti in vivo aplikovan\u00E9ho kolchicinu, vedouc\u00ED k v\u00FDskytu mixoploidn\u00EDch regenerant\u016F. P\u0159emno\u017Een\u00E9 DH regeneranty byly zahrnuty do test\u016F agronomick\u00FDch charakteristik v poln\u00EDch podm\u00EDnk\u00E1ch."@cs . . . . "Efektivita mikrosporov\u00FDch kultur pro produkci dihaploid\u016F v r\u00E1mci sdru\u017Een\u00ED %22\u010Cesk\u00E1 \u0159epka%22"@cs .