. . . "6"^^ . "Mach\u00E1\u010Dkov\u00E1, Ivana" . . . "0" . . . "PL - Polsk\u00E1 republika" . . "The research activities in the Czech Republic are directed to increase effectiveness of breeding procedures in winter oilseed rape using innovated methods. Initial plant materials for breeding line and hybrid cultivars, original self-incompatible doubled haploid lines, improved CMS and male fertility restorer lines (Rf) for Ogu-INRA and Shaan 2 systems were obtained by means of doubled haploids. About 1000 microspore culture regenerants have been produced every year. Tests of disease resistance especially against Phoma lingam and Sclerotinia sclerotiorum have been carried out in laboratory and in the field conditions. Frost tolerance laboratory tests of rape plants are performed and a method of in vitro selection has been developed. Molecular methods based on PCR technique are used for identification of self-incompatible genotypes and for detection of materials with modified seed quality and disease resistance. Perspective lines and hybrids are evaluated in breeders' joint multi-location trials" . "Inovace \u0161lechtitelsk\u00FDch postup\u016F \u0159epky ozim\u00E9 pro tvorbu zlep\u0161en\u00FDch v\u00FDchoz\u00EDch materi\u00E1l\u016F s vyu\u017Eit\u00EDm biotechnologick\u00FDch metod v \u010Cesk\u00E9 republice"@cs . "3"^^ . . "[436622FF28F9]" . "Innovations in breeding procedures of winter oilseed rape for development of improved initial materials and the use of biotechnological methods in the Czech Republic"@en . . . "The research activities in the Czech Republic are directed to increase effectiveness of breeding procedures in winter oilseed rape using innovated methods. Initial plant materials for breeding line and hybrid cultivars, original self-incompatible doubled haploid lines, improved CMS and male fertility restorer lines (Rf) for Ogu-INRA and Shaan 2 systems were obtained by means of doubled haploids. About 1000 microspore culture regenerants have been produced every year. Tests of disease resistance especially against Phoma lingam and Sclerotinia sclerotiorum have been carried out in laboratory and in the field conditions. Frost tolerance laboratory tests of rape plants are performed and a method of in vitro selection has been developed. Molecular methods based on PCR technique are used for identification of self-incompatible genotypes and for detection of materials with modified seed quality and disease resistance. Perspective lines and hybrids are evaluated in breeders' joint multi-location trials"@en . "V\u00FDzkumn\u00E1 \u010Dinnost v \u010Cesk\u00E9 republice je zam\u011B\u0159ena na zv\u00FD\u0161en\u00ED efektivnosti \u0161lechtitelsk\u00FDch postup\u016F u ozim\u00E9 \u0159epky vyu\u017Eit\u00EDm inovovan\u00FDch metod. V\u00FDchoz\u00ED materi\u00E1ly pro \u0161lecht\u011Bn\u00ED liniov\u00FDch a hybridn\u00EDch odr\u016Fd, origin\u00E1ln\u00ED autoimkompatibiln\u00ED DH linie, CMS linie a obnovitel\u00E9 fertility (RF)se zlep\u0161enou kvalitou pro Ogu-INRA a Shaan 2 CMS syst\u00E9my byly z\u00EDsk\u00E1ny pomoc\u00ED metody dihaploid\u016F. Ka\u017Ed\u00FDm rokem je produkov\u00E1no kolem 1000 mikrosporov\u00FDch regenerant\u016F. Testy rezistence v\u016F\u010Di chorob\u00E1m, zejm\u00E9na proti Phoma lingam a Sclerotinia sclerotiorum jsou prov\u00E1d\u011Bny v laboratorn\u00EDch a poln\u00EDch podm\u00EDnk\u00E1ch. Vyu\u017E\u00EDvaj\u00ED se laboratorn\u00ED testy mrazuvzdornosti a byla vyvinuta metoda in vitro selekce.Metody molekul\u00E1rn\u00ED genetiky zalo\u017Een\u00E9 na PCR jsou vyu\u017E\u00EDv\u00E1ny pro identifikaci autoinkompatibiln\u00EDch genotyp\u016F a pro detekci materi\u00E1l\u016F se zm\u011Bn\u011Bnou kvalitou semen a rezistenc\u00ED v\u016F\u010Di chorob\u00E1m. Perspektivn\u00ED linie a hybridy jsou hodnoceny v mezistani\u010Dn\u00EDch p\u0159edzkou\u0161k\u00E1ch p\u0159ed za\u0159azen\u00EDm do st\u00E1tn\u00EDch odr\u016Fdov\u00FDch zkou\u0161ek."@cs . . "Kl\u00EDma, Miroslav" . "0" . . "Vyvadilov\u00E1, Miroslava" . "P(1G46061)" . . . "RIV/00027006:_____/05:1242" . . "Innovations in breeding procedures of winter oilseed rape for development of improved initial materials and the use of biotechnological methods in the Czech Republic"@en . "Koprna, Radoslav" . "Innovations in breeding procedures of winter oilseed rape for development of improved initial materials and the use of biotechnological methods in the Czech Republic" . . . "1233-8273" . "oilseed rape, doubled haploids, hybrid breeding, self-incompatibility, CMS, disease resistance, frost resistance, molecular markers"@en . "Innovations in breeding procedures of winter oilseed rape for development of improved initial materials and the use of biotechnological methods in the Czech Republic" . "Ku\u010Dera, Vratislav" . . . "\u010Curn, Vladislav" . . "Inovace \u0161lechtitelsk\u00FDch postup\u016F \u0159epky ozim\u00E9 pro tvorbu zlep\u0161en\u00FDch v\u00FDchoz\u00EDch materi\u00E1l\u016F s vyu\u017Eit\u00EDm biotechnologick\u00FDch metod v \u010Cesk\u00E9 republice"@cs . "RIV/00027006:_____/05:1242!RIV07-MZE-00027006" . "525056" . "Rosliny oleiste" . . "12"^^ .