. "Produkce a vyu\u017Eit\u00ED dihaploid\u016F u zelenin druhu Brassica oleracea"@cs . . . . "Mo\u017Enost zv\u00FD\u0161en\u00ED efektivnosti regenerace rostlin z mikrosporov\u00FDch embry\u00ED vybran\u00FDch variet Brassica oleracea byla zkoum\u00E1na v letech 2001 a\u017E 2004. V\u00EDce ne\u017E 400 regenerant\u016F R1 generace bylo vytvo\u0159eno u kv\u011Bt\u00E1ku, kedlubnu a zel\u00ED pomoc\u00ED r\u016Fzn\u00FDch modifikac\u00ED mikrosporov\u00FDch technik. V\u00FDznamn\u00E9 rozd\u00EDly byly zaznamen\u00E1ny mezi genotypy v embryogenn\u00ED schopnosti a regeneraci z embry\u00ED do celistv\u00FDch rostlin. Nejvy\u0161\u0161\u00ED frekvence embryogeneze bylo dosa\u017Eeno u kv\u011Bt\u00E1ku cv. Siria, kedlubnu linie P7 a n\u011Bkter\u00FDch experiment\u00E1ln\u00EDch hybrid\u016F kv\u011Bt\u00E1ku. Nejlep\u0161\u00ED produkce embry\u00ED bylo dosa\u017Eeno kdy\u017E byly donorov\u00E9 rostliny p\u011Bstov\u00E1ny v kontrolovan\u00FDch podm\u00EDnk\u00E1ch v kultiva\u010Dn\u00EDch m\u00EDstnostech. Regenerace celistv\u00FDch rostlin byla v\u00FDznamn\u011B vy\u0161\u0161\u00ED po opakovan\u00E9 subkultivaci kotyledon\u00E1rn\u00EDch embry\u00ED na m\u00E9di\u00EDch s r\u016Fzn\u00FDmi kombinacemi fytohormon\u016F a s vyu\u017Eit\u00EDm od\u0159ez\u00E1v\u00E1n\u00ED \u010D\u00E1st\u00ED kotyledon\u016F u dosp\u011Bl\u00FDch embry\u00ED. Procento regenerace rostlin u kedlubnu se pohybovalo z 11,11 do 63,64 % a v p\u0159\u00EDpad\u011B zel\u00ED od 5,88 do 52,0 %. Vyu\u017Eit\u00ED regenerant\u016F pro produkci dihaploidn\u00EDch"@cs . "Production and utilization of doubled haploids in Brassica oleracea vegetables"@en . "RIV/00027006:_____/04:961!RIV08-MZE-00027006" . "Kl\u00EDma, Miroslav" . . "P(QD1356)" . . "Ku\u010Dera, Vratislav" . . "Produkce a vyu\u017Eit\u00ED dihaploid\u016F u zelenin druhu Brassica oleracea"@cs . "3"^^ . . "Horticultural Science (Prague)" . "119;123" . . . "3"^^ . . . "[BC2B94C8C375]" . "0862-867X" . "RIV/00027006:_____/04:961" . "A possibility to increase the efficiency of plant regeneration from microspore-derived embryos of selected botanical varieties of Brassica oleracea was investigated from 2001 to 2004. More than 400 regenerants of R1 generation were derived in kohlrabi, cabbage and cauliflower by means of different modifications of microspore culture technique. Distinct genotype differences in embryogenic responsibility and regenerative ability of microspore embryos to whole plants were detected. The highest frequency of embryogenesis and subsequent regeneration of plants were achieved in cauliflower Cultivar Siria Fl , kohlrabi line P7 and some experimental Fl hybrids of cauliflower. The best production of embryos was obtained when donor plants were grown in the growth chamber under controlled light and temperature conditions. The regeneration of plantlets was considerably improved by repeated subculture of cotyledonary embryos on media with various combinations of phytohormones and excision of the cotyledons from ma"@en . . "5"^^ . . "Vyvadilov\u00E1, Miroslava" . . "Production and utilization of doubled haploids in Brassica oleracea vegetables"@en . "582434" . "A possibility to increase the efficiency of plant regeneration from microspore-derived embryos of selected botanical varieties of Brassica oleracea was investigated from 2001 to 2004. More than 400 regenerants of R1 generation were derived in kohlrabi, cabbage and cauliflower by means of different modifications of microspore culture technique. Distinct genotype differences in embryogenic responsibility and regenerative ability of microspore embryos to whole plants were detected. The highest frequency of embryogenesis and subsequent regeneration of plants were achieved in cauliflower Cultivar Siria Fl , kohlrabi line P7 and some experimental Fl hybrids of cauliflower. The best production of embryos was obtained when donor plants were grown in the growth chamber under controlled light and temperature conditions. The regeneration of plantlets was considerably improved by repeated subculture of cotyledonary embryos on media with various combinations of phytohormones and excision of the cotyledons from ma" . "4" . "CZ - \u010Cesk\u00E1 republika" . "Production and utilization of doubled haploids in Brassica oleracea vegetables" . "31" . . "Brassica oleracea; microspore culture; plant regeneration; doubled haploids"@en . "Production and utilization of doubled haploids in Brassica oleracea vegetables" . . .