"161;173" . "Fluorescence-based AFLPs occur as the most suitable marker system for oiIseed rape cultivar identification" . . "1234-1983" . "Ovesn\u00E1, Jaroslava" . "Testovali jsme t\u0159i typy DNA mark\u00E9r\u016F - RAPD, SSR a AFLP mark\u00E9ry zalo\u017Een\u00E9 na fluorescen\u010Dn\u00EDm zna\u010Den\u00ED na jejich schopnost odli\u0161it genetick\u00E9 zdroje \u0159epky. Prok\u00E1zali jsem \u017Ee i u t\u00E9to plodiny AFLP je z veden\u00FDch mark\u00E9r\u016F nej\u00FA\u010Dinn\u011Bj\u0161\u00ED."@cs . . . "Sobotka, R." . "Fluorescence-based AFLPs occur as the most suitable marker system for oiIseed rape cultivar identification" . "564614" . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "45" . "Journal of Applied Genetics" . . "Z(MZE0002700602)" . "Three different types of molecular markers, RAPD, SSR and fluorescence-based AFLP, were evaluated and compared for their ability to identify oilseed rape cultivars. The direct comparison of RAPD, SSR and AFLP approaches in cultivar identification showed lhal the AFLP methodology detected polymorphisms more efficiently than either RAPD or SSR methods. For the characterisation of six oilseed rape cultivars, 60 RAPD primers were tested and only eight of them (14%) detected sufficient levels of polymorphism. Five microsatellites out of fifteen tested were polymorphic, but in a111oci, except one, on1y two different alleles were detected. This resu1t indicated the limited degree of polymorphism found in Brassica napus. Each of the six tested AFLP combinations detected polymorphisms, the best combination (M-CAA/E-ACT) had 26% polymorphic peaks from a total of90 peaks and could distinguish the analysed cultivars and 4 out of 5 core lines of cultivars. The results presented show that florescence-based AFLP is" . "Fluorescence-based AFLPs occur as the most suitable marker system for oiIseed rape cultivar identification"@en . "13"^^ . "RIV/00027006:_____/04:83!RIV08-MZE-00027006" . "Three different types of molecular markers, RAPD, SSR and fluorescence-based AFLP, were evaluated and compared for their ability to identify oilseed rape cultivars. The direct comparison of RAPD, SSR and AFLP approaches in cultivar identification showed lhal the AFLP methodology detected polymorphisms more efficiently than either RAPD or SSR methods. For the characterisation of six oilseed rape cultivars, 60 RAPD primers were tested and only eight of them (14%) detected sufficient levels of polymorphism. Five microsatellites out of fifteen tested were polymorphic, but in a111oci, except one, on1y two different alleles were detected. This resu1t indicated the limited degree of polymorphism found in Brassica napus. Each of the six tested AFLP combinations detected polymorphisms, the best combination (M-CAA/E-ACT) had 26% polymorphic peaks from a total of90 peaks and could distinguish the analysed cultivars and 4 out of 5 core lines of cultivars. The results presented show that florescence-based AFLP is"@en . . "RIV/00027006:_____/04:83" . "\u010Curn, V." . . "4"^^ . . "2" . . . "AFLP; rapeseed identification; diversity"@en . . . "1"^^ . "Dolansk\u00E1, L." . . "AFLP zalo\u017Een\u00E9 na fluorescenci je nejvhodn\u011Bj\u0161\u00EDm markerovac\u00EDm syst\u00E9mem pro identifikaci odr\u016Fd \u0159epky"@cs . "AFLP zalo\u017Een\u00E9 na fluorescenci je nejvhodn\u011Bj\u0161\u00EDm markerovac\u00EDm syst\u00E9mem pro identifikaci odr\u016Fd \u0159epky"@cs . . "Fluorescence-based AFLPs occur as the most suitable marker system for oiIseed rape cultivar identification"@en . "[A26AC0B935FF]" . . .