. "4"^^ . "The polymerase chain reaction (PCR) assay was successfully used to identify Cydia pomonella granulovirus (CpGV) in larvae of Cydia pomonella L. (codling moth). PCR with the primers CpGV-2A1/CpGV-2B and CpGV-3A/CpGV-3B was found suitable for detection of CpGV. The primers Cp-I/Cp-II and Cp-III/Cp-IV were able to identify the transposable element TCp3.2 in C. pomonella larvae. The presence of CpGV in the larvae from orchards, which had been infected with CpGV was tested during 2 years post infection. (pi.). CpGV was found in as many as 15% of the surviving larvae 1 year p.i. in one location. The virus was not detected in CpGV-infected orchards 2 years p.i. or in natural C. pomonella populations. This result suggests a poor persistence of CpGV in surviving C. pomonella individuals and its slow spread in a natural host population. One the other band, the presence of a transposable element, transposon TCp3.2 may correlate with virus redistribution in this insect population." . "Polymerase chain reaction assay for Cydia pomonella granulovirus detection on Cydia pomonella population" . "Kumar, Jiban" . . "Kocourek, Franti\u0161ek" . . "The polymerase chain reaction (PCR) assay was successfully used to identify Cydia pomonella granulovirus (CpGV) in larvae of Cydia pomonella L. (codling moth). PCR with the primers CpGV-2A1/CpGV-2B and CpGV-3A/CpGV-3B was found suitable for detection of CpGV. The primers Cp-I/Cp-II and Cp-III/Cp-IV were able to identify the transposable element TCp3.2 in C. pomonella larvae. The presence of CpGV in the larvae from orchards, which had been infected with CpGV was tested during 2 years post infection. (pi.). CpGV was found in as many as 15% of the surviving larvae 1 year p.i. in one location. The virus was not detected in CpGV-infected orchards 2 years p.i. or in natural C. pomonella populations. This result suggests a poor persistence of CpGV in surviving C. pomonella individuals and its slow spread in a natural host population. One the other band, the presence of a transposable element, transposon TCp3.2 may correlate with virus redistribution in this insect population."@en . "Polymerase chain reaction assay for Cydia pomonella granulovirus detection on Cydia pomonella population" . . "153;157" . "Cydia pomonella; codling moth; Cydia pomonella granulovirus; PCR; transposon"@en . "Metody PCR pro detekci Cydia pomonella granulovirus v populaci Cydia pomonella"@cs . "621522" . "Polymerase chain reaction assay for Cydia pomonella granulovirus detection on Cydia pomonella population"@en . "Metody PCR pro detekci Cydia pomonella granulovirus v populaci Cydia pomonella"@cs . . "Z(MZE-M01-01-03)" . "Pultar, O." . . "RIV/00027006:_____/03:33" . "Polymerase chain reaction assay for Cydia pomonella granulovirus detection on Cydia pomonella population"@en . "Acta Virologica" . . "0001-723X" . "3" . . "[34B900369EC6]" . . . "5"^^ . "Star\u00E1, Jitka" . . . . "SK - Slovensk\u00E1 republika" . . . "47" . . "PCR pro detekci granuloviru Cydia pomonella (CpGV) v larv\u00E1ch Cydia pomonella L. (obale\u010D jable\u010Dn\u00FD) byla vyvinuta. D\u00E1le byly vyvinuty primery pro detekci transposonu TCp3.2 v larv\u00E1ch C. pomonella."@cs . "RIV/00027006:_____/03:33!RIV08-MZE-00027006" . . "3"^^ . . .