"1"^^ . "7"^^ . "\u010Curn, Vladislav" . "Four protein fractions: 1 \u2013 albumins and globulins, 2 \u2013 gliadins, 3 \u2013 glutenins (extracted in NaOH), 4 \u2013 glutenins (extracted in SDS) separated by SDS-PAGE were used as biochemical markers for evaluation of polymorphism level in three spelt wheat cultivars \u2013 Hercule, Altgold a Rouquin, three new\u2013breeders\u2019 spelt lines \u2013 H92.27, H92.28 a M92.20 (originated from hybridisation between spelt and common wheat) and reference common wheat cultivar Brea. Electrophoretic phenotypes and zymograms were evaluated by means of digital image analysis and Nei and Li coefficient of similarity was used to evaluate the relation of analysed genotypes. Entire evaluation of all four-marker systems showed differences between common wheat cultivar Brea and spelt cultivars and spelt breeders\u2019 lines. Also significant differences between old spelt cultivars (Hercule, Altgold and Rouquin) and new spelt breeders\u2019 lines were found. The reality of the mutual passing of protein fractions (gliadins and glutenins), based on Osborne e"@en . "Hodnocen\u00ED b\u00EDlkovinn\u00FDch frakc\u00ED jako biochemick\u00FDch marker\u016F pro identifikaci odr\u016Fd p\u0161enice \u0161paldy (Triticum spelta L.)"@cs . . "Evaluation of protein fractions as biochemical markers for identification of spelt wheat cultivars (Triticum spelta L.)"@en . . "RIV/00027006:_____/03:1588!RIV08-GA0-00027006" . "99;105" . "Plant, Soil and Environment" . . "[4AB953524CCA]" . "Evaluation of protein fractions as biochemical markers for identification of spelt wheat cultivars (Triticum spelta L.)" . . "2"^^ . "spelt wheat, biochemical markers, seed proteins, electrophoresis, protein fractions"@en . . "\u010Cty\u0159i b\u00EDlkovinn\u00E9 frakce (1 \u2013 albuminy a globuliny), 2 \u2013 gliadiny, 3 \u2013 gluteniny (extrahovan\u00E9 pomoc\u00ED NaOH), 4 \u2013 gluteniny (extrahovan\u00E9 pomoc\u00ED SDS) byly d\u011Bleny v podm\u00EDnk\u00E1ch SDS na polyakrylamidov\u00E9m gelu a pou\u017Eity jako biochemick\u00E9 markery pro hodnocen\u00ED stupn\u011B polymorfismu u t\u0159\u00ED odr\u016Fd p\u0161enice \u0161paldy \u2013 Hercule, Altgold a Rouquin a t\u0159\u00ED novo\u0161lecht\u011Bn\u00ED \u2013 H92.27, H92.28 a M92.20 (vytvo\u0159en\u00FDch k\u0159\u00ED\u017Een\u00EDm p\u0161enice \u0161paldy s p\u0161enic\u00ED setou) a referen\u010Dn\u00ED odr\u016Fdy p\u0161enice set\u00E9 Brea. V\u00FDsledn\u00E9 elektroforeogramy byly digitalizov\u00E1ny a pomoc\u00ED Nei a Li koeficientu podobnosti byl vyhodnocen vztah mezi sledovan\u00FDmi genotypy. Celkov\u00E9 hodnocen\u00ED v\u0161ech \u010Dty\u0159 markerovac\u00EDch syst\u00E9m\u016F vykazovalo diference mezi p\u0161enic\u00ED setou Brea, odr\u016Fdami \u0161paldy i hybridn\u00EDmi liniemi. Rovn\u011B\u017E byly zji\u0161t\u011Bny v\u00FDznamn\u00E9 diference mezi p\u016Fvodn\u00EDmi star\u00FDmi odr\u016Fdami p\u0161enice \u0161paldy (Hercule, Altgold a Rouquin) a hybridn\u00EDmi liniemi p\u0161enice \u0161paldy. V podm\u00EDnk\u00E1ch Osbornovy extrakce byla potvrzena skute\u010Dnost vz\u00E1jemn\u00E9ho p\u0159echodu proteinov\u00FDch frakc\u00ED (gliadin\u016F a glutenin\u016F). Z"@cs . "CZ - \u010Cesk\u00E1 republika" . "49" . "RIV/00027006:_____/03:1588" . "Evaluation of protein fractions as biochemical markers for identification of spelt wheat cultivars (Triticum spelta L.)" . . . . . . "3" . . . "Dvo\u0159\u00E1\u010Dek, V\u00E1clav" . "1214-1178" . . "Hodnocen\u00ED b\u00EDlkovinn\u00FDch frakc\u00ED jako biochemick\u00FDch marker\u016F pro identifikaci odr\u016Fd p\u0161enice \u0161paldy (Triticum spelta L.)"@cs . . "Four protein fractions: 1 \u2013 albumins and globulins, 2 \u2013 gliadins, 3 \u2013 glutenins (extracted in NaOH), 4 \u2013 glutenins (extracted in SDS) separated by SDS-PAGE were used as biochemical markers for evaluation of polymorphism level in three spelt wheat cultivars \u2013 Hercule, Altgold a Rouquin, three new\u2013breeders\u2019 spelt lines \u2013 H92.27, H92.28 a M92.20 (originated from hybridisation between spelt and common wheat) and reference common wheat cultivar Brea. Electrophoretic phenotypes and zymograms were evaluated by means of digital image analysis and Nei and Li coefficient of similarity was used to evaluate the relation of analysed genotypes. Entire evaluation of all four-marker systems showed differences between common wheat cultivar Brea and spelt cultivars and spelt breeders\u2019 lines. Also significant differences between old spelt cultivars (Hercule, Altgold and Rouquin) and new spelt breeders\u2019 lines were found. The reality of the mutual passing of protein fractions (gliadins and glutenins), based on Osborne e" . "Evaluation of protein fractions as biochemical markers for identification of spelt wheat cultivars (Triticum spelta L.)"@en . . . "606425" . "P(GA303/01/1380)" . .