"Brdi\u010Dka, Radim" . "Merkerov\u00E1, Michaela" . . "P(NR7989)" . "454162" . "RIV/00023736:_____/07:00007110!RIV07-MZ0-00023736" . . "34" . "27;33" . . "We tested a variety of physical and chemical non-vector based transfection methods in order to evaluate which of them gave the most suitable transfer of siRNA into chronic myeloid leukemia (CML) primary cells. Chemically synthesized siRNAs against several genes were transfected into the cells and level of knockdown was determined by qRT-PCR. According to our results, nucleofection (Amaxa) appears to be the only suitable non-viral method for siRNA delivery into the hard-to-transfect CML primary cells"@en . . . . "12"^^ . . . "Targeting of gene expression by siRNA in CML primary cells"@en . "Za \u00FA\u010Delem vybr\u00E1n\u00ED nejvhodn\u011Bj\u0161\u00EDho postupu vn\u00E1\u0161en\u00ED siRNA molekul do prim\u00E1rn\u00EDch bun\u011Bk chronick\u00E9 myeloidn\u00ED leukemie (CML) byla testov\u00E1na \u0161k\u00E1la fyzik\u00E1ln\u00EDch a chemick\u00FDch non-vektorov\u00FDch transfek\u010D\u00EDch metod. Chemicky p\u0159ipraven\u00E9 siRNA proti n\u011Bkolika gen\u016Fm byly transfekov\u00E1ny do bun\u011Bk a \u00FA\u010Dinnost transfekce byla ur\u010Dena pomoc\u00ED qRT-PCR. Na z\u00E1klad\u011B na\u0161ich v\u00FDsledk\u016F bylo zji\u0161t\u011Bno, \u017Ee nukleofekce (Amaxa) je jedinou \u00FA\u010Dinnou metodou pro transfer siRNA do t\u011B\u017Eko transfekovateln\u00FDch CML bun\u011Bk"@cs . . . "4"^^ . . . . "Targeting of gene expression by siRNA in CML primary cells" . "NL - Nizozemsko" . "RIV/00023736:_____/07:00007110" . "4"^^ . "We tested a variety of physical and chemical non-vector based transfection methods in order to evaluate which of them gave the most suitable transfer of siRNA into chronic myeloid leukemia (CML) primary cells. Chemically synthesized siRNAs against several genes were transfected into the cells and level of knockdown was determined by qRT-PCR. According to our results, nucleofection (Amaxa) appears to be the only suitable non-viral method for siRNA delivery into the hard-to-transfect CML primary cells" . . . "[A02A16C392E7]" . "Tlumen\u00ED genov\u00E9 exprese pomoc\u00ED siRNA u CML prim\u00E1rn\u00EDch bun\u011Bk"@cs . . "Targeting of gene expression by siRNA in CML primary cells" . . "chemical transfection; CML; electroporation; nucleofection; siRNA; primary cells"@en . "Bruchov\u00E1, Hana" . . . . "Klamov\u00E1, Hana" . "Molecular Biology Reports" . "Tlumen\u00ED genov\u00E9 exprese pomoc\u00ED siRNA u CML prim\u00E1rn\u00EDch bun\u011Bk"@cs . . "1" . "Targeting of gene expression by siRNA in CML primary cells"@en . "0301-4851" .