"468897" . "P(GA203/05/0038), P(GA303/04/0432), P(LC531), V, Z(AV0Z40550506)" . "Cloning and characterization of Sapp2p, the second aspartic proteinase isoenzyme from Candida parapsilosis" . "RIV/00023736:_____/06:00006659" . "6" . . . . . . "NL - Nizozemsko" . . "Pichov\u00E1, I." . "Candida parapsilosis possesses 3 genes encoding secreted aspartic proteinases. The Sapp2p precursor was expressed in E. coli and purified. Autoactivation of pro-Sapp2p was inefficient and resulted in a protein extended by 8 amino acids at the N-terminus. The correct promature junction was cleaved by trypsin or by a membrane-bound Kex2-like proteinase from C. parapsilosis. The mature Sapp2p was proteolytically active and its substrate specificity differed from that of Sapp1p."@en . "Candida parapsilosis possesses 3 genes encoding secreted aspartic proteinases. The Sapp2p precursor was expressed in E. coli and purified. Autoactivation of pro-Sapp2p was inefficient and resulted in a protein extended by 8 amino acids at the N-terminus. The correct promature junction was cleaved by trypsin or by a membrane-bound Kex2-like proteinase from C. parapsilosis. The mature Sapp2p was proteolytically active and its substrate specificity differed from that of Sapp1p." . "Cloning and characterization of Sapp2p, the second aspartic proteinase isoenzyme from Candida parapsilosis"@en . "Dost\u00E1l, J." . . "Klonov\u00E1n\u00ED a charakterizace Sapp2p, druh\u00E9ho izoenzymu aspart\u00E1tov\u00E9 proteiasy z Candida parapsilosis"@cs . . . "Hradilek, M." . "Hru\u0161kov\u00E1-Heidingsfeldov\u00E1, O." . "FEMS yeast research" . . "Candida parapsilosis obsahuje 3 geny k\u00F3duj\u00EDc\u00ED sekretovan\u00E9 aspart\u00E1tov\u00E9 prote\u00E1zy. Prekursor prote\u00E1zy Sapp2p byl exprimov\u00E1n v E. coli a purifikov\u00E1n. Autoaktivace Sapp2p byla m\u00E1lo \u00FA\u010Dinn\u00E1 a vedla ke vzniku proteinu prodlou\u017Een\u00E9ho na N-konci o 8 aminokyselin. K aktivaci v o\u010Dek\u00E1van\u00E9m m\u00EDst\u011B do\u0161lo \u00FA\u010Dinkem trypsinu nebo membr\u00E1nov\u00E9 prote\u00E1zy Kex2 z C. parapsilosis. Maturn\u00ED Sapp2p byla proteolyticky aktivn\u00ED a jej\u00ED substr\u00E1tov\u00E1 specifita se li\u0161ila od specifity Sapp1p."@cs . . . . . "1567-1356" . "RIV/00023736:_____/06:00006659!RIV09-MZ0-00023736" . "5"^^ . "Cloning and characterization of Sapp2p, the second aspartic proteinase isoenzyme from Candida parapsilosis" . . "9"^^ . "Merkerov\u00E1, Michaela" . "Klonov\u00E1n\u00ED a charakterizace Sapp2p, druh\u00E9ho izoenzymu aspart\u00E1tov\u00E9 proteiasy z Candida parapsilosis"@cs . "[85B39138B0AB]" . . "Cloning and characterization of Sapp2p, the second aspartic proteinase isoenzyme from Candida parapsilosis"@en . . "1"^^ . "000241200400009" . "7" . "Candida parapsilosis; aspartic proteinase"@en . . .