. "4" . . . "P\u00E1len\u00ED\u010Dkov\u00E1, Eli\u0161ka" . "5"^^ . . . "lysosomal lipase; high-fat diet; high-sucrose diet; steatosis; rat"@en . "CZ - \u010Cesk\u00E1 republika" . "RIV/00023001:_____/10:00002388" . "This study was designed to test the role of liver lipases in the degradation of liver triacylglycerols (TAG) and to determine the effect of dietary induced TAG accumulation in the liver on regulation of their lipolysis. Male Wistar rats were administered high-fat or high-sucrose diet for two weeks. Individual lipases (HL; TGH; LAL) were identified according to their different pH optimum. Administration of both diets resulted in liver TAG accumulation (HFD >>> HSD). The only lipase capable to hydrolyse intracellular TAG was LAL. On standard diet, LAL activity towards both endogenous and exogenous substrates was up-regulated in fasting and down-regulated in fed state. The intensity of autophagy determined according to the LC3-II/LC3-I protein ratio followed a similar pattern. HFD led to an increase of this ratio, elevation of LAL activity in phagolysosomal fraction and abolishment of fasting/fed-dependent differences. LAL activity significantly correlated with ketogenesis in all groups (r = 0.8"@en . . "Folia biologica (Praha)" . "Kazdov\u00E1, Ludmila" . "5"^^ . . "P(NS9696), Z(MZ0IKEM2005)" . "248258" . . "0015-5500" . . "Da\u0148kov\u00E1, Helena" . "The autophagy-lysosomal pathway is involved in TAG degradation in the liver: the effect of high-sucrose and high-fat diet"@en . "Pap\u00E1\u010Dkov\u00E1, Zuzana" . . "This study was designed to test the role of liver lipases in the degradation of liver triacylglycerols (TAG) and to determine the effect of dietary induced TAG accumulation in the liver on regulation of their lipolysis. Male Wistar rats were administered high-fat or high-sucrose diet for two weeks. Individual lipases (HL; TGH; LAL) were identified according to their different pH optimum. Administration of both diets resulted in liver TAG accumulation (HFD >>> HSD). The only lipase capable to hydrolyse intracellular TAG was LAL. On standard diet, LAL activity towards both endogenous and exogenous substrates was up-regulated in fasting and down-regulated in fed state. The intensity of autophagy determined according to the LC3-II/LC3-I protein ratio followed a similar pattern. HFD led to an increase of this ratio, elevation of LAL activity in phagolysosomal fraction and abolishment of fasting/fed-dependent differences. LAL activity significantly correlated with ketogenesis in all groups (r = 0.8" . . . . "The autophagy-lysosomal pathway is involved in TAG degradation in the liver: the effect of high-sucrose and high-fat diet" . "000281924500005" . . "10"^^ . . . . "RIV/00023001:_____/10:00002388!RIV11-MZ0-00023001" . "[56CC52AA4657]" . . . . "56" . "The autophagy-lysosomal pathway is involved in TAG degradation in the liver: the effect of high-sucrose and high-fat diet" . . . . "Cahov\u00E1, Monika" . "The autophagy-lysosomal pathway is involved in TAG degradation in the liver: the effect of high-sucrose and high-fat diet"@en .