"Highly sensitive method for quantitative determination of bilirubin in biological fluids and tissues"@en . . . "Highly sensitive method for quantitative determination of bilirubin in biological fluids and tissues"@en . "Nekonjugovan\u00FD bilirubin m\u00E1 antioxida\u010Dn\u00ED a cytoprotektivn\u00ED \u00FA\u010Dinky, ve vysok\u00FDch koncentrac\u00EDch v\u0161ak vyvol\u00E1v\u00E1 apopt\u00F3zu a je cytotoxick\u00FD. P\u0159esn\u00E9 m\u011B\u0159en\u00ED bilirubinu ve tk\u00E1n\u00EDch je nezbytn\u00E9 ke studiu jeho \u00FAlohy v redoxn\u00EDch regulac\u00EDch, prevenci ateroskler\u00F3zy a malignit. V na\u0161\u00ED studii jsme vyvinuli a validovali vysoce citlivou chromatografickou metodu detekce tk\u00E1\u0148ov\u00E9ho bilirubinu. V\u00FDsledky m\u011B\u0159en\u00ED ve tk\u00E1n\u00EDch Gunnov\u00FDch potkan\u016F byly srovnateln\u00E9 s publikovan\u00FDmi hodnotami nam\u011B\u0159en\u00FDmi jin\u00FDmi technikami."@cs . . "bilirubin; Gunn rats; HPLC; method of determination; tissue bilirubin"@en . . "10"^^ . "Kudla, Michal" . "1" . "Bal\u00E1\u017E, Peter" . "Highly sensitive method for quantitative determination of bilirubin in biological fluids and tissues" . . . . . "P(ME 849), Z(MZ0IKEM2005)" . "RIV/00023001:_____/08:00001760" . "Unconjugated bilirubin (UCB) exhibits potent antioxidant and cytoprotective properties, but causes apoptosis and cytotoxicity at pathologically elevated concentrations. Accurate measurement of UCB concentrations in cells, fluids and tissues is needed to evaluate its role in redox regulation, prevention of atherosclerotic and malignant diseases, and bilirubin encephalopathy. In the present study, we developed and validated a highly sensitive method for tissue UCB determinations. UCB was extracted from rat organs with chloroform/methanol/hexane at pH 6.2 and then partitioned into a minute volume of alkaline buffer that was subjected to HPLC using an octyl reverse phase (RP) column. Addition of mesobilirubin as an internal standard corrected for losses of UCB during extraction. Recoveries averaged 75+/-5%. The detection limit was 10pmol UCB/g wet tissue. Variance was +/-2.5%. When used to measure UCB concentrations in tissues of jaundiced Gunn rats, this procedure yielded UCB levels directly comparable t" . . "RIV/00023001:_____/08:00001760!RIV09-MZ0-00023001" . "370121" . . "Muchov\u00E1, L." . "000256200400007" . . "Vysoce citliv\u00E1 metoda pro stanoven\u00ED bilirubinu v biologick\u00FDch tekutin\u00E1ch a tk\u00E1n\u00EDch"@cs . "6"^^ . . . "NL - Nizozemsko" . "Wong, R. J." . . . . . . "Jirsa, Milan" . "867" . . "Ostrow, J. D." . "3"^^ . "Len\u00ED\u010Dek, M." . "Highly sensitive method for quantitative determination of bilirubin in biological fluids and tissues" . "[05EF0220B6F6]" . . . "Unconjugated bilirubin (UCB) exhibits potent antioxidant and cytoprotective properties, but causes apoptosis and cytotoxicity at pathologically elevated concentrations. Accurate measurement of UCB concentrations in cells, fluids and tissues is needed to evaluate its role in redox regulation, prevention of atherosclerotic and malignant diseases, and bilirubin encephalopathy. In the present study, we developed and validated a highly sensitive method for tissue UCB determinations. UCB was extracted from rat organs with chloroform/methanol/hexane at pH 6.2 and then partitioned into a minute volume of alkaline buffer that was subjected to HPLC using an octyl reverse phase (RP) column. Addition of mesobilirubin as an internal standard corrected for losses of UCB during extraction. Recoveries averaged 75+/-5%. The detection limit was 10pmol UCB/g wet tissue. Variance was +/-2.5%. When used to measure UCB concentrations in tissues of jaundiced Gunn rats, this procedure yielded UCB levels directly comparable t"@en . "Vysoce citliv\u00E1 metoda pro stanoven\u00ED bilirubinu v biologick\u00FDch tekutin\u00E1ch a tk\u00E1n\u00EDch"@cs . "Zelenka, J." . "V\u00EDtek, L." . "Journal of Chromatography B : Analytical technologies in the biomedical and life sciences" . "Zadinov\u00E1, M." . "1570-0232" .