"Konzumace alkoholu ovliv\u0148uje expresi genu pro lipoproteinovou lip\u00E1zu u C57BL/6 my\u0161\u00ED"@cs . "2"^^ . . "5"^^ . "420518" . "Physiological Research" . "Konzumace alkoholu ovliv\u0148uje expresi genu pro lipoproteinovou lip\u00E1zu u C57BL/6 my\u0161\u00ED"@cs . "[35040D5F21C5]" . . . "2"^^ . "apolipoprotein A-I; lipoprotein lipase; ethanol consumption; gene expression; mouse"@en . . . "Ethanol consumption affects lipoprotein lipase gene expression in C57BL/6 mice" . "The activity of lipoprotein lipase (LPL) is increased after alcohol consumption and can contribute to an increased level of HDL-cholesterol, which is considered to play a key role in the ethanol-mediated protective effect against cardiovascular disease. The increase in HDL-cholesterol concentration can be also due to an ethanol-enhanced synthesis and secretion of apolipoprotein A-I (apo A-I) from hepatocytes. Therefore, the hypothesis that ethanol consumption affects the LPL and apo A-I gene (LPL and APOA1, respectively) expression was tested in male C57BL/6 mice drinking 5 % ethanol or water and fed a standard chow or high-fat (HF) diet for 4 weeks. The LPL expression was determined in the heart, epididymal and dorsolumbal adipose tissues, the APOA1 expression in the liver. Alcohol consumption did not affect lipid and lipoprotein concentrations in the serum. The LPL expression was increased in the heart of mice given ethanol and HF diet compared to mice on chow and ethanol (p<0.001) and was also i" . . . "RIV/00023001:_____/07:00000753!RIV08-MZ0-00023001" . "Mudr\u00E1kov\u00E1, Eli\u0161ka" . "RIV/00023001:_____/07:00000753" . . . . "Ethanol consumption affects lipoprotein lipase gene expression in C57BL/6 mice" . . . "56" . "Ethanol consumption affects lipoprotein lipase gene expression in C57BL/6 mice"@en . . . "Ethanol consumption affects lipoprotein lipase gene expression in C57BL/6 mice"@en . . "The activity of lipoprotein lipase (LPL) is increased after alcohol consumption and can contribute to an increased level of HDL-cholesterol, which is considered to play a key role in the ethanol-mediated protective effect against cardiovascular disease. The increase in HDL-cholesterol concentration can be also due to an ethanol-enhanced synthesis and secretion of apolipoprotein A-I (apo A-I) from hepatocytes. Therefore, the hypothesis that ethanol consumption affects the LPL and apo A-I gene (LPL and APOA1, respectively) expression was tested in male C57BL/6 mice drinking 5 % ethanol or water and fed a standard chow or high-fat (HF) diet for 4 weeks. The LPL expression was determined in the heart, epididymal and dorsolumbal adipose tissues, the APOA1 expression in the liver. Alcohol consumption did not affect lipid and lipoprotein concentrations in the serum. The LPL expression was increased in the heart of mice given ethanol and HF diet compared to mice on chow and ethanol (p<0.001) and was also i"@en . "P(NR7847)" . . . "0862-8408" . "4" . "Aktivita lipoproteinov\u00E9 lip\u00E1zy (LPL) je zv\u00FD\u0161ena po um\u00EDrn\u011Bn\u00E9 konzumaci alkoholu a m\u016F\u017Ee p\u0159isp\u00EDvat ke zv\u00FD\u0161en\u00E9 hladin\u011B HDL-cholesterolu, kter\u00E1 hraje kl\u00ED\u010Dovou \u00FAlohu v protektivn\u00EDm \u00FA\u010Dinku alkoholu proti kardiovaskul\u00E1rn\u00EDm onemocn\u011Bn\u00EDm. Zv\u00FD\u0161en\u00E1 hladina HDL-cholesterolu m\u016F\u017Ee b\u00FDt t\u00E9\u017E zp\u016Fsobena t\u00EDm, \u017Ee alkohol stimuluje synt\u00E9zu a sekreci apolipoproteinu A-I (apo A-I) v j\u00E1trech. U samc\u016F my\u0161\u00ED C57BL/6 jsme proto testovali hypot\u00E9zu, \u017Ee konzumace alkoholu m\u00E1 vliv na expresi gen\u016F pro LPL a apo A-I (LPL a APOA1). My\u0161i pily vodu nebo 5% alkohol a byly krmeny standardn\u00ED laboratorn\u00ED dietou nebo dietou vysokotukovou (HF) po dobu 4 t\u00FDdn\u016F. Exprese LPL byla stanovena v srdci, epididym\u00E1ln\u00ED a dorsolumb\u00E1ln\u00ED tukov\u00E9 tk\u00E1ni, exprese APOA1 v j\u00E1trech. Konzumace alkoholu nem\u011Bla vliv na koncentraci lipid\u016F a lipoprotein\u016F v s\u00E9ru. Exprese LPL byla zv\u00FD\u0161ena v srdci my\u0161\u00ED pij\u00EDc\u00EDch etanol na HF diet\u011B ve srovn\u00E1n\u00ED s t\u011Bmi na alkoholu a standardn\u00ED diet\u011B (p<0,001) a tak\u00E9 v epididym\u00E1ln\u00EDm tuku u my\u0161\u00ED pij\u00EDc\u00EDch alkohol a krmen\u00FDch HF dietou proti my\u0161\u00EDm"@cs . "497-501" . "Kov\u00E1\u0159, Jan" . "CZ - \u010Cesk\u00E1 republika" . .