"Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5\u03B1 in Bioaerosols" . . "RIV/00000493:G41__/11:#0000388!RIV12-MO0-G41_____" . . . . "Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5\u03B1 in Bioaerosols"@en . . . "Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5\u03B1 in Bioaerosols" . . . "The portable detector ImmunoSMART was combined with the cyclone air sampler to detect the model bioagent E. coli DH5\u03B1 in bioaerosols. The selected capture antibody was immobilized on gold electrodes, antibody-peroxidase conjugate served as a tracer. In buffer, the amperometric immunosensor measured 103 to 108\u2005CFU/mL within 30\u2005min. Detection in air was realized in a closed aerosol chamber. Automated dissemination of the cells, sampling and measurement allowed remote testing of the cyclone/immunodetector. The level of 150\u2005CFU/L in air was clearly indicated (20\u2005min analysis including 5\u2005min sampling) and independently confirmed using the slit sampler and agar cultivation. The results seem promising for further extended tests in both aerosol chamber and field trial conditions." . . "Bioagents; Sandwich immunoassay; Biosensors; Remote sensing; Aerosol chamber"@en . "3" . "1040-0397" . "The portable detector ImmunoSMART was combined with the cyclone air sampler to detect the model bioagent E. coli DH5\u03B1 in bioaerosols. The selected capture antibody was immobilized on gold electrodes, antibody-peroxidase conjugate served as a tracer. In buffer, the amperometric immunosensor measured 103 to 108\u2005CFU/mL within 30\u2005min. Detection in air was realized in a closed aerosol chamber. Automated dissemination of the cells, sampling and measurement allowed remote testing of the cyclone/immunodetector. The level of 150\u2005CFU/L in air was clearly indicated (20\u2005min analysis including 5\u2005min sampling) and independently confirmed using the slit sampler and agar cultivation. The results seem promising for further extended tests in both aerosol chamber and field trial conditions."@en . "[1295AE0E9F10]" . "8"^^ . "G41" . "7"^^ . . . . "Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5\u03B1 in Bioaerosols"@en . "Electroanalysis" . "24" . . . "RIV/00000493:G41__/11:#0000388" . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . "\u0160v\u00E1bensk\u00E1, Eva" . "2"^^ . . "197034" . . "P(OVVTUO2008001)" . . "Skl\u00E1dal, Petr" . .