. "http://www.isvav.cz/projectDetail.do?rowId=VF20122015024"^^ . "2012-04-20+02:00"^^ . "2015-12-31+01:00"^^ . . . . "VF20122015024" . . . . . . "C\u00EDlem projektu je vypracovat metodick\u00E9 postupy pro izolaci bakteri\u00E1ln\u00EDch a virov\u00FDch nukleov\u00FDch kyselin a proteinov\u00FDch a neproteinov\u00FDch toxin\u016F z p\u0159irozen\u00FDch matric, a d\u00E1le pak postupy pro jejich identifikaci a typizaci. V p\u0159\u00EDpad\u011B bakteri\u00ED a vir\u016F jsou navr\u017Eeny n\u00E1sleduj\u00EDc\u00ED metodick\u00E9 postupy a specifick\u00E9 technologick\u00E9 a laboratorn\u00ED celky: z\u00EDsk\u00E1v\u00E1n\u00ED a kultivace biologick\u00FDc agens, izolace genomov\u00E9 a plasmidov\u00E9 DNA, resp. RNA v p\u0159\u00EDpad\u011B vir\u016F, metodiky a postupy p\u0159\u00EDpravy vzork\u016F bakteri\u00E1ln\u00EDch a virov\u00FDch nukleov\u00FDch kyselin z komplexn\u00EDch matric, n\u00E1vrh qPCR primer\u016F, sond, reak\u010Dn\u00EDch podm\u00EDnek a lad\u011Bn\u00ED protokol\u016F a otestov\u00E1n\u00ED a validace navr\u017Een\u00FDch metod a postup\u016F pro identifikaci biologick\u00FDch agens. Pro detekci n\u00EDzkomolekul\u00E1rn\u00EDch toxin\u016F bude pou\u017Eita vysoko\u00FA\u010Dinn\u00E1 kapalinov\u00E1 chromatografie ve spojen\u00ED s tandemovou hmotnostn\u00ED spektrometri\u00ED a pro detekci a identifikaci proteinov\u00FDch toxin\u016F bude pou\u017Eita hmotnostn\u011B spektrometrick\u00E1 metoda SRM (Selected reaction monitoring)." . "2014-09-29+02:00"^^ . . "1"^^ . "Bacteria, viruses, toxins, nucleic acids, PCR, liquid chromatography, mass spectrometry"@en . "0"^^ . . . "0"^^ . "2"^^ . . "0"^^ . "New technologies for identification and typing of biological agents"@en . "Nov\u00E9 technologie identifikace a typizace biologick\u00FDch agens" . . " viruses" . "Bacteria" . . " toxins" . "The aim of the project is to develop the methodological procedures for the isolation of bacterial and viral nucleic acids and protein and no-protein toxins from natural matrices, and the procedures for their identification and typing. In the case of bacteria and viruses are designed following the methodological procedures and specific technological and laboratory units: the acquisition and cultivation of biological agents, isolation of genome and plasmid DNA, or RNA in the case of a virus, the methodology and procedures for the preparation of samples of bacterial and viral nucleic acids from complex matrices, design of qPCR primers, probes and the reaction conditions and testing and validation of the proposed methods and procedures for the identification of biological agents. For the detection of low molecular weight toxins will be used high performance liquid chromatography coupled with tandem mass spectrometry and for detection and identification of protein toxins will be used mass spectrometric method SRM(Selected reaction monitoring)."@en . . . "2015-03-25+01:00"^^ . . " PCR" . " nucleic acids" . " liquid chromatography" .