" quantitative real-time PCR" . . "2008-01-01+01:00"^^ . . "C\u00EDlem bylo z\u00EDskat nov\u00E9 poznatky o genetick\u00E9 diversit\u011B je\u010Dmene a zav\u00E9st metodu stanoven\u00ED kvantitativn\u00ED exprese se zam\u011B\u0159en\u00EDm na geny ovliv\u0148uj\u00EDc\u00ED obsah fenolick\u00FDch l\u00E1tek. Poskytnout tak n\u00E1stroj c\u00EDlen\u00E9ho vyu\u017Eit\u00ED genov\u00FDch zdroj\u016F je\u010Dmene ve \u0161lecht\u011Bn\u00ED a u\u017Eit\u00ED produkce."@cs . . " biodiversity" . . "The goal was to obtain novel findings on the genetic diversity of barley and to establish a method of determining quantitative expression aimed at genes influencing the phenolic compound content. This results in a tool for the targeted use of genetic sources of barley for cultivation and the use of production."@en . "2013-10-16+02:00"^^ . . . "http://www.isvav.cz/projectDetail.do?rowId=QH82277"^^ . . . . "2012-03-02+01:00"^^ . . . " gene expression" . "Z\u00EDskat nov\u00E9 poznatky o genetick\u00E9 diversit\u011B je\u010Dmene a zav\u00E9st metodu stanoven\u00ED kvantitativn\u00ED exprese se zam\u011B\u0159en\u00EDm na geny ovliv\u0148uj\u00EDc\u00ED obsah fenolick\u00FDch l\u00E1tek. Poskytnout tak n\u00E1stroj c\u00EDlen\u00E9ho vyu\u017Eit\u00ED genov\u00FDch zdroj\u016F je\u010Dmene ve \u0161lecht\u011Bn\u00ED a u\u017Eit\u00ED produkce." . "1"^^ . . . . " polyphenoloxidase" . . "0"^^ . "Studium diverzity vybran\u00FDch polyfenoloxid\u00E1z je\u010Dmene ve vztahu ke kvalit\u011B a stabilit\u011B produkce." . "2012-12-31+01:00"^^ . "3"^^ . . . "barley; biodiversity; polyphenoloxidase; quantitative real-time PCR; gene expression; malting quality"@en . "6"^^ . . "Study of barley polyphenoloxidase diversity in relation to quality and production stability"@en . "6"^^ . "Within the conducted project, original and priority knowledge about a gene structure for selected oxidoreductases (polyphenoloxidases) in barley on a level of DNA sequences and/or their allelic forms in the barley collection with characterized traits and properties will be obtained. Then, sets of diagnostic primers for detection of diversity in examined genes will be designed and verified that are required for genotyping and selection of gene resources. Furthermore, methods for quantitative assessment of expression of the examined genes using qRT-PCR on barley grain and plants will be proposed and applied. A special collection of gene resources with characterized traits and selected properties (agronomic, nutritional and malting) affecting production quality and use will be created."@en . . "barley" . "QH82277" . .