. "paratuberculosis" . "Within this project, new approaches for the detection and typing of the causative agent of paratuberculosis using culture and molecular biology methods base on the PCR will be introduced. The project will trace sources and distribution of M. a. paratuberculosis and analyse the risks and transmission ways among domestic and wild living ruminants. In selected herds, infection intensity of excrements (solid and liquid manure etc.) from animals infected with the causative agent of paratuberculosis, its survival and risk of contamination of fertilized soil and pastures will be investigated. In the experiments, potential penetration of the causative agent of paratuberculosis via root system into the tissues of vegetables cultivated in infected soil will be assessed. Within evaluation of milk and milk product, contamination risks during their processing, presence of live, sublethally damaged or dead cells of the causative agent of paratuberculosis in biofilms prepared in vitro and biofilms from selected dair"@en . " DNA chip" . . " milk products" . " ecology" . . "QH81065" . " ruminants" . . " Mycobacterium avium subspecies paratuberculosis" . " biofilm" . . . . "3"^^ . "0"^^ . "C\u00EDlem projektu bylo prov\u011B\u0159it zdroje M. a. paratuberculosis a prok\u00E1zat v\u00FDskyt a p\u0159e\u017E\u00EDv\u00E1n\u00ED v p\u016Fd\u011B, hnojivech, rostlin\u00E1ch, v ml\u00E9ce a ml\u00E9\u010Dn\u00FDch v\u00FDrobc\u00EDch a v biofilmech ml\u00E9\u010Dn\u00FDch provoz\u016F. Pro tyto \u00FA\u010Dely pou\u017E\u00EDt st\u00E1vaj\u00EDc\u00ED metody kultivace, PCR a RFLP a vyvinout nov\u00E9 postupy. Pl\u00E1novan\u00E9 v\u00FDstupy byly spln\u011Bny."@cs . "Prov\u011B\u0159it zdroje M. a. paratuberculosis a prok\u00E1zat v\u00FDskyt a p\u0159e\u017E\u00EDv\u00E1n\u00ED v p\u016Fd\u011B, hnojivech, rostlin\u00E1ch, v ml\u00E9ce a ml\u00E9\u010Dn\u00FDch v\u00FDrobc\u00EDch a v biofilmech ml\u00E9\u010Dn\u00FDch provoz\u016F. Pro tyto \u00FA\u010Dely pou\u017E\u00EDt st\u00E1vaj\u00EDc\u00ED metody kultivace, PCR a RFLP a vyvinout nov\u00E9 postupy." . . "2012-03-02+01:00"^^ . . " real time PCR" . "2013-10-16+02:00"^^ . . "Suppression of paratuberculosis in the CZ: introduction of new methods for the causative agent detection, monitoring of its survival and spread in studs, its distribution in the environment and examination of food and feed contamination."@en . "1"^^ . "2008-01-01+01:00"^^ . . "paratuberculosis; real time PCR; DNA chip; ruminants; milk; milk products; Mycobacterium avium subspecies paratuberculosis; biofilm; ecology; excrements"@en . "Tlumen\u00ED paratuberkul\u00F3zy v \u010Cesk\u00E9 republice: zaveden\u00ED nov\u00FDch metod pro urychlen\u00ED detekce p\u016Fvodce, sledov\u00E1n\u00ED jeho p\u0159e\u017E\u00EDv\u00E1n\u00ED a \u0161\u00ED\u0159en\u00ED v chovech, jeho distribuce v prost\u0159ed\u00ED a posouzen\u00ED rizik kontaminace krmiv a potravin" . . "The project aim was to verify the M. paratuberculosis sources and to demonstrate its presence and survival in soil, fertilizers, plants, milk and dairy products as well as in the biofilm present in dairy operations. The existing cultivation methods and the PCR and RFLP methods were used to attain this project objective and new procedures were developed. The outputs anticipated were fulfilled."@en . "2012-12-31+01:00"^^ . . . "39"^^ . "39"^^ . . . . . . "http://www.isvav.cz/projectDetail.do?rowId=QH81065"^^ . . " milk" .