"Instability of the genome of bone marrow cells of patients with AML and MDS."@en . "0"^^ . . "2012-10-31+01:00"^^ . "2010-05-01+02:00"^^ . . . . "1"^^ . . "0"^^ . "2012-02-27+01:00"^^ . "The aim of our study in cooperation with COST projecBM0801 is to analyse chromosomal changes in complex karyotypes of patients with MDS and AML in detail using all molecular cytogenetic techniques which are available and to search for the new, so far non-described recurrent chromosomal changes. We wish to define the frequency and significance of these aberrations for disease progression and transformation of MDS into AML. As many rearrangements are cryptic and therefore their identification is possible by molecular-cytogenetic methods only, we will exploit modifications of FISH methods routinely used in our Center of Oncocytogenetics (FISH, I-FISH. mFISH, mBAND, CGH) and as the new approach we will start to use array CGH analyses. We will start pilot study with the specially designed chip for array CGH analyses of chromosome 5. We will also continue to search for rearrangments of MLL gene in myeloid malignancies to establish prognostic values in our cohort. We have very close co-operation with clin"@en . "2013-06-28+02:00"^^ . . . "OC10043" . "7"^^ . "Zjistit nen\u00E1hodnost vstupu chromosom\u016F do p\u0159estaveb a rekurenci zlomov\u00FDch m\u00EDst. Identifikovat geny spolup\u016Fsob\u00EDc\u00ED p\u0159i progresi malignity a geny v oblastech s delecemi, zejm\u00E9na na chromosomu 5. Definovat skupiny pacient\u016F vhodn\u00E9 pro individu\u00E1ln\u00ED l\u00E9\u010Dbu." . "genome instability in malignant cells chromosomal aberrations molecular cytogenetics myelodysplastic syndromes (MDS) acute myeloid leukemia (AML)"@en . "Celogenomovou anal\u00FDzou jsme u souboru nemocn\u00FDch s MDS/AML a komplexn\u00EDm karyotypem ur\u010Dili frekvenci rekurentn\u00EDch nebalancovan\u00FDch aberac\u00ED. Popsali jsme 375 zlomov\u00FDch m\u00EDst lokalizovan\u00FDch ve zn\u00E1m\u00FDch fragiln\u00EDch m\u00EDstech. Popsali jsme atypick\u00E9 delece 5q, kter\u00E9 nezahrnovaly oblast CDR. Potvrdili jsme nestabilitu deletovan\u00E9ho chromosomu 5, kter\u00FD vstupuje do p\u0159estaveb s r\u016Fzn\u00FDmi partnersk\u00FDmi chromosomy."@cs . "7"^^ . . . "Nestabilita genomu bun\u011Bk kostn\u00ED d\u0159en\u011B nemocn\u00FDch s MDS a AML" . . . . . . . . . . "By whole-genome analysis we determined the frequency of recurrent unbalanced aberrations in patients with MDS/AML and complex karyotype. We described 375 breakpoints located at known fragile sites as well as atypical 5q deletions that did not include the CDR. We confirmed substantial instability of d chromosome 5, often entering other rearrangements with various partner chromosomes."@en . "http://www.isvav.cz/projectDetail.do?rowId=OC10043"^^ . .