. "DNA methylation status of selected genes was correlated with patients' prognosis and mutational status of DNMT3A gene. We found influence of DNA methylation on overall survival and incidence of relapses as well as relationship between DNMT3A mutations and DNA methylation. Further we performed whole genome bisulfite sequencing and expression profiling to reveal the complex role of DNA methylation."@en . . " minimal residual disease" . . . . "2013-06-28+02:00"^^ . . . "Metyla\u010Dn\u00ED status vybran\u00FDch gen\u016F byl korelov\u00E1n s progn\u00F3zou onemocn\u011Bn\u00ED a s muta\u010Dn\u00EDm stavem genu DNMT3A. Nalezli jsme souvislost mezi metylac\u00ED DNA t\u011Bchto gen\u016F, celkov\u00FDm p\u0159e\u017Eit\u00EDm a incidenc\u00ED relaps\u016F. D\u00E1le jsme detekovali ni\u017E\u0161\u00ED metylac\u00ED DNA u pacient\u016F s mutovan\u00FDm genem DNMT3A. Pro komplexn\u00ED pohled na zm\u011Bny v metylaci DNA bylo provedeno celogenomov\u00E9 bisulfitov\u00E9 sekvenov\u00E1n\u00ED a expresn\u00ED profilov\u00E1n\u00ED."@cs . "Application of DNA methylation arrays for assessment of aberrantly methylated genes in MDS and AML patients"@en . " epigenetics" . "Hematological malignancies, such as other malignancies, have specific epigenetic alterations of tumor suppressor genes, genes involved in differentiation, apoptosis, metastasis etc. The alterations of gene promoter methylation lead to uncontrolled proliferation of leukemic cells and malignant transformation. The DNA methylation changes can be used to detect leukemic cells in peripheral blood. We will examine the DNA methylation status of a wide spectrum of genes by the use of DNA methylation PCR arrays which allows us to observe multiple changes in gene methylation profile. These arrays are convenient for a more detail insight of epigenetic modifications occurring during the malignant transformation of myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML). We will choose the panel of the most promising genes based on our previous results from cultivation with demethylating agents such as Decitabine and on recently published data from genome-wide methylation studies. We will assess the"@en . "http://www.isvav.cz/projectDetail.do?rowId=OC10042"^^ . " DNA methylation array" . "Na z\u00E1klad\u011B na\u0161ich p\u0159edb\u011B\u017En\u00FDch v\u00FDsledk\u016F a \u00FAdaj\u016F z literatury chceme z\u00EDskat nov\u00E9 informace o epigenetick\u00E9 regulaci leukemogeneze AML a progrese MDS a t\u00EDm p\u0159isp\u011Bt k individualizaci a zv\u00FD\u0161en\u00ED \u00FA\u010Dinnosti l\u00E9\u010Dby AML a MDS. (max.255 znak\u016F)" . "hypermethylation; tumor suppressor genes; epigenetics; minimal residual disease; demethylation drugs; DNA methylation array; MDS; AML"@en . . . . . "2012-11-30+01:00"^^ . . . . "hypermethylation" . "2012-02-27+01:00"^^ . " MDS" . "OC10042" . . " demethylation drugs" . . "1"^^ . "Studium aberantn\u011B metylovan\u00FDch gen\u016F u pacient\u016F s MDS a AML pomoc\u00ED DNA metyla\u010Dn\u00EDch array\u00ED." . "0"^^ . "1"^^ . . "1"^^ . "2010-05-01+02:00"^^ . . . " tumor suppressor genes" . . "0"^^ .