. "1"^^ . "0"^^ . "1"^^ . "2015-12-31+01:00"^^ . . "CML; BCR-ABL1; HMMR/RHAMM; AURKA; ESPL1; separase; RT-Q-PCR; antibodies; DNA vaccines; helper epitope; xenogeneic genes; localization signals"@en . "Search for prognostic markers of chronic myelogenous leukemia (CML) and development of DNA vaccines against this disease based on increased production of centrosomal proteins. Detection of HMMR/RHAMM, AURKA and ESPL1 mRNA level in blood cells and antibodies against the corresponding proteins in CML patients at disease diagnosis and during subsequent monitoring. Utilization of the obtained findings for optimization of therapy. Development of DNA vaccines against HMMR and AURKA that should be efficient against CML stem cells resistant to imatinib. Enhancement of vaccine immunogenicity by the addition of cell localization signals and sequences encoding strong helper epitopes. Analysis of the effect of xenogeneic origin of the genes on vaccine immunogenicity. Reduction of HMMR and AURKA potencial oncogenicity by the mutagenesis of functional domains and by the removal of their initiation codons in fusion genes. Observation of immune responses induced with the developed DNA vaccines in mouse models."@en . "0"^^ . " AURKA" . " HMMR/RHAMM" . . . "2012-04-01+02:00"^^ . "Utilization of centrosomal proteins for prognostication and vaccinotherapy of chronic myeloid leukemia"@en . " DNA vaccines" . " RT-Q-PCR" . . " xenogeneic genes" . "2015-01-22+01:00"^^ . . . . "Hled\u00E1n\u00ED prognostick\u00FDch ukazatel\u016F u chronick\u00E9 myeloidn\u00ED leukemie (CML) a v\u00FDvoj DNA vakc\u00EDn proti tomuto onemocn\u011Bn\u00ED zalo\u017Een\u00FDch na zv\u00FD\u0161en\u00E9 produkci centrozom\u00E1ln\u00EDch protein\u016F. Stanoven\u00ED hladiny mRNA gen\u016F HMMR/RHAMM, AURKA a ESPL1 v krevn\u00EDch bu\u0148k\u00E1ch a protil\u00E1tek proti p\u0159\u00EDslu\u0161n\u00FDm protein\u016Fm v s\u00E9rech pacient\u016F s CML p\u0159i diagn\u00F3ze onemocn\u011Bn\u00ED a b\u011Bhem n\u00E1sledn\u00E9ho monitoringu. Mo\u017Enost vyu\u017Eit\u00ED z\u00EDskan\u00FDch poznatk\u016F k optimalizaci l\u00E9\u010Dby, kter\u00E1 by mohla zahrnovat terapeutickou imunizaci. V\u00FDvoj DNA vakc\u00EDn proti HMMR a AURKA, kter\u00E9 by m\u011Bly b\u00FDt \u00FA\u010Dinn\u00E9 i proti kmenov\u00FDm bu\u0148k\u00E1m CML rezistentn\u00EDm k l\u00E9\u010Db\u011B imatinibem. Zv\u00FD\u0161en\u00ED imunogennosti vakc\u00EDn p\u0159id\u00E1n\u00EDm bun\u011B\u010Dn\u00FDch lokaliza\u010Dn\u00EDch sign\u00E1l\u016F a sekvenc\u00ED z genu tetanov\u00E9ho toxinu k\u00F3duj\u00EDc\u00EDch pomocn\u00E9 epitopy. Anal\u00FDza vlivu xenogenn\u00EDch gen\u016F na imunogennost vakc\u00EDn. Sn\u00ED\u017Een\u00ED potenci\u00E1ln\u00ED onkogennosti HMMR a AURKA mutagenez\u00ED funk\u010Dn\u00EDch dom\u00E9n a odstran\u011Bn\u00EDm jejich inicia\u010Dn\u00EDch kodon\u016F u f\u00FAzn\u00EDch gen\u016F. Stanoven\u00ED imunitn\u00EDch reakc\u00ED vyvolan\u00FDch vyvinut\u00FDmi protin\u00E1dorov\u00FDmi DNA vakc\u00EDnami na my\u0161\u00EDch modelech." . . . " helper epitope" . . . "CML" . "http://www.isvav.cz/projectDetail.do?rowId=NT13862"^^ . " antibodies" . . . " ESPL1" . "2014-03-21+01:00"^^ . " separase" . "Vyu\u017Eit\u00ED centrozom\u00E1ln\u00EDch protein\u016F pro prognostiku a vakcinoterapii chronick\u00E9 myeloidn\u00ED leukemie" . . . "NT13862" . "1"^^ . " BCR-ABL1" . .