"2014-03-21+01:00"^^ . . " cytotoxicity" . . "V r\u00E1mci projektu budou p\u0159ipraveny a charakterizov\u00E1ny prim\u00E1rn\u00ED kultury lidsk\u00FDch hepatocyt\u016F s vyu\u017Eit\u00EDm jatern\u00ED tk\u00E1n\u011B z\u00EDskan\u00E9 od d\u00E1rc\u016F org\u00E1n\u016F, pro kter\u00E9 nebyl nalezen vhodn\u00FD p\u0159\u00EDjemce. Prim\u00E1rn\u00ED kultury budou vyu\u017Eity pro studium jatern\u011B specifick\u00FDch transkrip\u010Dn\u011B regula\u010Dn\u00EDch mechanism\u016F biotransforma\u010Dn\u00EDch enzym\u016F, zejm\u00E9na pak cytochrom\u016F P450. Z\u00EDskan\u00E9 v\u00FDsledky umo\u017En\u00ED pochopit (a p\u0159edv\u00EDdat a p\u0159edch\u00E1zet) mezil\u00E9kov\u00E9 interakce, p\u0159edev\u0161\u00EDm v r\u00E1mci imunosupresivn\u00EDch protokol\u016F, a chemicky vyvolanou hepatokarcinogenezi." . . "Prim\u00E1rn\u00ED kultury lidsk\u00FDch hepatocyt\u016F jako model pro toxikologick\u00E9, farmakologick\u00E9 a biochemick\u00E9 studie v transplanta\u010Dn\u00ED medic\u00EDn\u011B" . . . . . . . . . " drug metabolism" . "http://www.isvav.cz/projectDetail.do?rowId=NT13591"^^ . "human hepatocytes" . "2012-04-01+02:00"^^ . "2015-12-31+01:00"^^ . "2015-01-22+01:00"^^ . "2"^^ . . "2"^^ . "1"^^ . "2"^^ . "Primary cultures of human hepatocytes will be prepared and characterized using liver tissue obtained from multiorgan donors, for whom suitable recipient is not available. Primary cultures will be used for the study of liver-specific transcriptional regulatory mechanisms of biotransformation enzymes, especially cytochrome P450. The obtained results will allow to understand (and predict and prevent) the interactions between drugs especially in the context of immunosuppressive protocols and chemically induced hepatocarcinogenesis"@en . . " drug interactions" . "human hepatocytes; cytotoxicity; drug interactions; drug metabolism; hepatic physiology"@en . "0"^^ . . . "NT13591" . . . "Primary cultures of human hepatocytes as a model for toxicological, pharmacological and biochemical studies in transplantation medicine."@en . . .