. "Mutations in p53 are associated with particularly poor prognosis in chronic lymphocytic leukemia. These harmful mutations are frequently selected by conventional therapy, which leads to fatal consequences. Their early identification - before fully penetrant selection - could assign corresponding patients for alternative treatment approaches giving them chance to survive. We intent to use the next-generation sequencing technology with the extreme sensitivity of mutation detection (1-2% of affected cells) and to analyze: (a) original samples from patients, which developed later (after therapy) p53 mutation detectable by standard techniques; (b) original samples from patients, which stayed p53 wild-type after therapy administration. The aim is to establish a system for as early identification of p53 mutations as possible and avoid use of conventional therapy in patients affected by minor proportion p53 mutation."@en . " TP53/p53" . . "NT13519" . "http://www.isvav.cz/projectDetail.do?rowId=NT13519"^^ . . . "2012-04-01+02:00"^^ . "0"^^ . "2015-12-31+01:00"^^ . "\u010Casn\u00E1 identifikace CLL pacient\u016F s dosud nevyselektovan\u00FDmi mutacemi v proteinu p53" . "2014-03-21+01:00"^^ . "Mutace v proteinu p53 jsou asociov\u00E1ny s obzvl\u00E1\u0161t\u011B \u0161patnou progn\u00F3zou u chronick\u00E9 lymfocyt\u00E1rn\u00ED leuk\u00E9mie. Tyto \u0161kodliv\u00E9 mutace jsou \u010Dasto selektov\u00E1ny konven\u010Dn\u00ED terapi\u00ED, co\u017E vede k fat\u00E1ln\u00EDm n\u00E1sledk\u016Fm. Jejich \u010Dasn\u00E1 identifikace - p\u0159ed plnou selekc\u00ED - by mohla vy\u010Dlenit odpov\u00EDdaj\u00EDc\u00ED pacienty pro alternativn\u00ED l\u00E9\u010Debn\u00E9 postupy, kter\u00E9 by poskytly nad\u011Bji na p\u0159e\u017Eit\u00ED. Zam\u00FD\u0161l\u00EDme pou\u017E\u00EDt technologii %22next-generation sequencing%22 s extr\u00E9mn\u00ED citlivost\u00ED detekce mutac\u00ED (1-2% posti\u017Een\u00FDch bun\u011Bk) a analyzovat: (a) p\u016Fvodn\u00ED vzorky od pacient\u016F, u kter\u00FDch se po l\u00E9\u010Db\u011B vyvinula p53 mutace detekovateln\u00E1 standardn\u00EDmi technikami; (b) p\u016Fvodn\u00ED vzorky od pacient\u016F, kter\u00E9 z\u016Fstaly p53 \u201Ewild-type\u201C po aplikaci terapie. C\u00EDlem je etablovat diagnostick\u00FD syst\u00E9m pro tak \u010Dasnou identifikaci p53 mutac\u00ED jak bude mo\u017En\u00E9 a p\u0159edej\u00EDt pou\u017Eit\u00ED konven\u010Dn\u00ED terapie u pacient\u016F s minoritn\u00EDm p53 mutovan\u00FDm klonem." . "Chronic lymphocytic leukemia" . . . "1"^^ . . . . "1"^^ . . . . " next-generation sequencing" . . . . "Early identification of CLL patients with yet unselected p53 mutation"@en . . "6"^^ . "6"^^ . "2015-01-22+01:00"^^ . " mutation selection" . . "Chronic lymphocytic leukemia; TP53/p53; mutation selection; next-generation sequencing; early detection"@en . .