"Vztah mezi mno\u017Estv\u00EDm bakteri\u00ED v plodov\u00E9 vod\u011B a intenzitou intraamni\u00E1ln\u00ED z\u00E1n\u011Btliv\u00E9 odpov\u011Bdi u pacientek s p\u0159ed\u010Dasn\u00FDm odtokem plodov\u00E9 vody" . . "2012-04-01+02:00"^^ . . "1"^^ . "U pacientek s p\u0159ed\u010Dasn\u00FDm odtokem plodov\u00E9 vody bude ve vzorc\u00EDch plodov\u00E9 vody odebran\u00E9 transabdomin\u00E1ln\u00ED amniocent\u00E9zou stanovena: a) bakteri\u00E1ln\u00ED n\u00E1lo\u017E genit\u00E1ln\u00EDch mykoplazmat a Streptococcus agalactiae rozd\u00EDln\u00FDmi kvantifika\u010Dn\u00EDmi technikami. b) intenzita intraamni\u00E1ln\u00ED inflamatorn\u00ED odpov\u011Bdi na p\u0159\u00EDtomnost bakteri\u00ED v plodov\u00E9 vod\u011B stanoven\u00EDm vybran\u00FDch cytokin\u016F, chemokin\u016F a alarmin\u016F. Budou identifikov\u00E1ny \u201Ckritick\u00E9\u201D bakteri\u00E1ln\u00ED n\u00E1lo\u017Ee t\u011Bchto mikroorganizm\u016F v plodov\u00E9 vod\u011B zodpov\u011Bdn\u00E9 za rozvoj z\u00E1va\u017En\u00E9 intraamni\u00E1ln\u00ED infekce a z\u00E1n\u011Btu. Tento proces je z\u0159ejm\u011B z\u00E1visl\u00FD na gesta\u010Dn\u00EDm st\u00E1\u0159\u00ED, proto budou hodnoty stanoveny pro t\u0159i rozd\u00EDln\u00E9 periody t\u011Bhotenstv\u00ED mezi 24.-36. t\u00FDdnem gravidity. Klinick\u00E9 pozorov\u00E1n\u00ED budou verifikov\u00E1na na experiment\u00E1ln\u00EDm modelu vytvo\u0159en\u00E9m z plodov\u00FDch obal\u016F. Mo\u017Enost klinick\u00E9ho vyu\u017Eit\u00ED jednoduch\u00E9ho relativn\u00EDho kvantifika\u010Dn\u00EDho p\u0159\u00EDstupu ke stanoven\u00ED n\u00E1lo\u017Ee genit\u00E1ln\u00EDch mykoplazmat - threshold cycle (Ct) hodnoty z real-time PCR bude validov\u00E1na absolutn\u00ED kvantifikac\u00ED." . . . "2"^^ . . . "http://www.isvav.cz/projectDetail.do?rowId=NT13461"^^ . "quantification of bacteria" . . "5"^^ . "5"^^ . . . . "NT13461" . "2015-01-22+01:00"^^ . . "2014-03-21+01:00"^^ . . " molecular biology" . "The sample of amniotic fluid will be retrieval by transabdominal amniocentesis. The bacterial load of genital mycoplasmas/Streptococcus agalactiae with different quantification approach along with intensity of intraamniotic inflammatory response to bacteria by a panel of cytokines, chemokines and alarmins will be determined. The \u201Ccritical\u201D values of microbial burden responsible for exhibition of intensive intraamniotic infection and inflammation will be proposed. These values will be described for three different periods of pregnancy. Clinical observations will be verified with using of amnio-chorion explant model. The efficacy of threshold cycle value of real time PCR will be validated by absolute quantification approach."@en . "2015-12-31+01:00"^^ . . "0"^^ . . . " microbial load" . "quantification of bacteria; microbial load; molecular biology; preterm labor"@en . . "The relationship between bacterial load in amniotic fluid and the intensity of intraamniotic inflammatory response in women with preterm prelabor rupture of membranes"@en . .