. "2"^^ . "http://www.isvav.cz/projectDetail.do?rowId=NT13242"^^ . "2015-01-22+01:00"^^ . "biofilm" . "2012-04-01+02:00"^^ . "Poly-microbial biofilm - the cause of chronic urinary infections and therapy failure: cultivation and PCR-DGGE diagnostics, importance and possibilities of affection"@en . . . "Catheterization of patient is common during many interventions. This is one of reasons, why over 40% of nosocomial infections are infections of urinary tract, esp. of catheterized patients. Poly-microbial wound infection are known to be only partially cultivable, part of microbes can be proved only by molecular techniques. The microbial diversity of biofilm communities of urethral stents and catheters was not satisfactory examined. Suitable method for poly-microbial diagnostic is Denaturing Gradient Gel Electrophoresis (DGGE). In most studies the PCR-DGGE proved also non-cultivable bacteria and even bacteria unknown with those infections. The aim of this project is to study of species composition of biofilm communities by cultivation and molecular methods and assessment of the differences in poly-microbial communities in relation to therapy failure, length of insertion of catheter, its type and other factors including predominating microbial species."@en . . . . "2015-12-31+01:00"^^ . . . "NT13242" . . . " infection" . . . . " urinary catheter" . " CAUTI" . . . "Polymikrobi\u00E1ln\u00ED biofilm jako p\u0159\u00ED\u010Dina chronick\u00FDch mo\u010Dov\u00FDch infekc\u00ED a selh\u00E1v\u00E1n\u00ED terapie: kultiva\u010Dn\u00ED a PCR-DGGE diagnostika, v\u00FDznam a mo\u017Enosti \u0159e\u0161en\u00ED" . . "biofilm; urinary catheter; CAUTI; infection; DGGE"@en . "0"^^ . "Kat\u00E9trizace pat\u0159\u00ED k b\u011B\u017En\u00FDm postup\u016Fm p\u0159i \u0159ad\u011B z\u00E1krok\u016F, s \u010D\u00EDm\u017E souvis\u00ED fakt \u017Ee p\u0159es 40 % nozokomi\u00E1ln\u00EDch infekc\u00ED jsou pr\u00E1v\u011B inf. mo\u010Dov\u00E9ho traktu, zejm\u00E9na inf. kat\u00E9trizovan\u00FDch pacient\u016F. U polymikrobi\u00E1ln\u00EDch infekc\u00ED ran je zn\u00E1mo, \u017Ee kultiva\u010Dn\u011B lze zachytit pouze \u010D\u00E1st mikrob\u016F a \u010D\u00E1st lze prok\u00E1zat pouze metodami molekul\u00E1rn\u00EDmi. Mikrobi\u00E1ln\u00ED diverzita biofilmov\u00FDch spole\u010Denstev mo\u010Dov\u00FDch stent\u016F a kat\u00E9tr\u016F nebyla dosud uspokojiv\u011B prozkoum\u00E1na. Vhodnou metodou molekul\u00E1rn\u00ED anal\u00FDzy se zd\u00E1 denatura\u010Dn\u00ED gradientov\u00E1 gelov\u00E1 elektrofor\u00E9za (DGGE). V\u011Bt\u0161inou, kdy\u017E byla PCR-DGGE pou\u017Eita, byly zachyceny i bakterie nekultivovateln\u00E9, nav\u00EDc byly prok\u00E1z\u00E1ny bakterie u dan\u00FDch infekc\u00ED dosud nezn\u00E1m\u00E9. C\u00EDlem projektu je studium druhov\u00E9ho slo\u017Een\u00ED biofilmov\u00FDch spole\u010Denstev kultiva\u010Dn\u011B a molekul\u00E1rn\u00EDmi technikami a zhodnocen\u00ED rozd\u00EDl\u016F v polymikrobi\u00E1ln\u00EDch spole\u010Denstvech ve vztahu k selh\u00E1n\u00ED terapie, d\u00E9lce zaveden\u00ED kat\u00E9tru, jeho druhu a dal\u0161\u00EDm faktor\u016Fm v\u010Detn\u011B predominuj\u00EDc\u00EDch mikrobi\u00E1ln\u00EDch druh\u016F." . "2014-03-21+01:00"^^ . . "4"^^ . "4"^^ . "1"^^ .