. "In clinical part of the study the hypothesis that hypercholesterolemic patients - carriers of -203C variant in cholesterol 7alpha-hydroxylase (CYP7A1) gene respond to 4-week therapy with bile acid sequestrant by more pronounced decrease in cholesterolemia than -203A variant carriers will be tested. The dynamics of changes in concentration of cholesterol, lipoproteins, glucose and factors affecting insulin sensitivity induced by changes in bile acid metabolism after sequestrant administration will be studied in healthy volunteers. It will be also analyzed how the changes in bile acid metabolism affect glucoregulation and thyroid function. In molecular biology part of the project the dual luciferase assay will be used to analyze in detail the role of particular polymorphisms in promoter region and first intron of CYP7A1 gene (polymorphisms being in tight linkage disequilibrium with -203A/C polymorphism) in regulation of cholesterol-7alpha-hydroxylase gene expression."@en . . "cholesterol 7alpha-hydroxylase; genetics; cholesterol; bile acids; bile acid sequestrants; insulin sensitivity"@en . "0"^^ . "2014-03-21+01:00"^^ . . " bile acids" . "1"^^ . "0"^^ . "1"^^ . . "0"^^ . . " bile acid sequestrants" . . . . . "cholesterol 7alpha-hydroxylase" . . "2015-01-22+01:00"^^ . . . "2012-04-01+02:00"^^ . . . . "V klinick\u00E9 \u010D\u00E1sti projektu bude testov\u00E1na hypot\u00E9za, \u017Ee hypercholesterolemi\u010Dt\u00ED pacienti - nositel\u00E9 varianty -203C v genu k\u00F3duj\u00EDc\u00EDm cholesterol-7alfa-hydroxylasu (CYP7A1) odpov\u00EDdaj\u00ED na 4t\u00FDdenn\u00ED l\u00E9\u010Dbu sekvestranty \u017Elu\u010Dov\u00FDch kyselin podstatn\u011B v\u011Bt\u0161\u00EDm poklesem cholesterol\u00E9mie ne\u017E nositel\u00E9 varianty -203A. Dynamika zm\u011Bn koncentrace cholesterolu, lipoprotein\u016F, gluk\u00F3zy a faktor\u016F ovliv\u0148uj\u00EDc\u00EDch inzul\u00EDnosenzitivitu indukovan\u00FDch zm\u011Bnami v metabolismu \u017Elu\u010Dov\u00FDch kyselin po pod\u00E1n\u00ED sekvestrantu bude studov\u00E1na u zdrav\u00FDch dobrovoln\u00EDk\u016F. D\u00E1le bude analyzov\u00E1no, jak zm\u011Bny v metabolismu \u017Elu\u010Dov\u00FDch kyselin ovlivn\u00ED glykoregulaci a funkci \u0161t\u00EDtn\u00E9 \u017El\u00E1zy. V molekul\u00E1rn\u011B biologick\u00E9 \u010D\u00E1sti projektu bude metodou du\u00E1ln\u00EDho luciferasov\u00E9ho stanoven\u00ED detailn\u011B analyzov\u00E1na \u00FAloha polymorfism\u016F v promotorov\u00E9 oblasti a 1. intronu genu k\u00F3duj\u00EDc\u00EDho CYP7A1 v regulaci exprese cholesterol-7alfa-hydroxylasy." . . "http://www.isvav.cz/projectDetail.do?rowId=NT13151"^^ . "Cholesterol 7alpha-hydroxylase polymorphism as a predictor cholesterolemia responsiveness"@en . " cholesterol" . " genetics" . "NT13151" . . "2015-12-31+01:00"^^ . . "Polymorfismus cholesterol-7alfa-hydroxylasy jako prediktor odpov\u00EDdavosti cholesterol\u00E9mie" .