"2015-01-22+01:00"^^ . . . . "2012-04-01+02:00"^^ . "NT13099" . . . . . " serum" . " cultivation" . . "pancreatic islets" . "2015-12-31+01:00"^^ . "We will test new methods of protection and cultivation of isolated pancreatic islets designed for transplant therapy of diabetes using the following modifications: supplementation with blood serum in order to improve the cell vitality and to stop collagenase activity, prevention of apoptosis and undesirable cell dedifferantiation by specific inhibitors and the use of mesenchymal stem cells conditioned medium for islet culture. The results will be used as a basis for improvement of the current islet transplant protocol"@en . "Optimalization of the culture conditions of the isolated Langerhans islets for transplantation"@en . . "2014-03-21+01:00"^^ . "0"^^ . . "1"^^ . . "0"^^ . "http://www.isvav.cz/projectDetail.do?rowId=NT13099"^^ . . "0"^^ . "Optimalizace kultiva\u010Dn\u00EDch podm\u00EDnek izolovan\u00FDch Langerhnasov\u00FDch ostr\u016Fvk\u016F ur\u010Den\u00FDch k transplantaci" . . . "0"^^ . "Budeme testovat nov\u00E9 metody ochrany a kultivace izolovan\u00FDch Langerhansov\u00FDch ostr\u016Fvk\u016F ur\u010Den\u00FDch pro transplanta\u010Dn\u00ED l\u00E9\u010Dbu diabetu. Tyto metody jsou zalo\u017Een\u00E9 na pou\u017Eit\u00ED st\u00E1vaj\u00EDc\u00EDho kultiva\u010Dn\u00EDho m\u00E9dia dopln\u011Bn\u00E9ho o kompatibiln\u00ED krevn\u00ED s\u00E9rum, inhibitory apopt\u00F3zy,stimul\u00E1tory replikace beta bun\u011Bk a blok\u00E1tory ne\u017E\u00E1douc\u00ED dediferenciace a d\u00E1le na pou\u017Eit\u00ED nov\u00E9ho kultiva\u010Dn\u00EDho m\u00E9dia a m\u00E9dia kondiciovan\u00E9ho mesenchym\u00E1lnymi kmenov\u00FDmi bu\u0148kami. V\u00FDsledky budou slou\u017Eit jako z\u00E1klad pro zm\u011Bnu klinick\u00E9ho protokolu izolace a kultivace Langerhansov\u00FDch ostr\u016Fvk\u016F ur\u010Den\u00FDch k transplantaci." . . "pancreatic islets; serum; cultivation; transplantation"@en . . .