. . . "2011-06-01+02:00"^^ . "prostate cancer; circulating tumor cells; gene expression profiling"@en . " circulating tumor cells" . "0"^^ . . "2"^^ . "2015-12-31+01:00"^^ . "2"^^ . "Detekce cirkuluj\u00EDc\u00EDch n\u00E1dorov\u00FDch bun\u011Bk a sledov\u00E1n\u00ED exprese gen\u016F u kastra\u010Dn\u011B rezistentn\u00EDho karcinomu prostaty jako sou\u010D\u00E1st individualizace syst\u00E9mov\u00E9 l\u00E9\u010Dby" . . "1"^^ . . "prostate cancer" . . . . "0"^^ . "NT12205" . . . . . "http://www.isvav.cz/projectDetail.do?rowId=NT12205"^^ . "C\u00EDlem projektu je detekovat cirkuluj\u00EDc\u00ED n\u00E1dorov\u00E9 bu\u0148ky (CTC) v perifern\u00ED krvi u pacient\u016F s metastatick\u00FDm karcinomem prostaty p\u0159ed cytotoxickou l\u00E9\u010Dbou a v jej\u00EDm pr\u016Fb\u011Bhu. Detekce bude provedena metodou imunomagnetick\u00E9 separace bun\u011Bk a izolac\u00ED RNA z t\u00E9to frakce. D\u00E1le prob\u011Bhne kultivace \u010D\u00E1sti detekovan\u00FDch bun\u011Bk v\u010Detn\u011B kultivace v prost\u0159ed\u00ED cytotoxick\u00FDch l\u00E1tek. V dal\u0161\u00ED f\u00E1zi bude hodnocen genetick\u00FD profil zji\u0161t\u011Bn\u00FDch bun\u011Bk (stanoven\u00ED panelu 24 gen\u016F) a obdobn\u00FD profil tak\u00E9 u histologick\u00E9ho prepar\u00E1tu prim\u00E1rn\u00EDho tumoru. V\u00FDsledky anal\u00FDz budou vzta\u017Eeny na klinick\u00FD pr\u016Fb\u011Bh onemocn\u011Bn\u00ED a budou stanoveny prognostick\u00E9 faktory \u00FA\u010Dinku l\u00E9\u010Dby na pokles CTC a p\u0159e\u017Eit\u00ED pacient\u016F." . . "The aim of the project is to detect circulating tumor cells (CTC) in peripheral blood of patients with metastatic prostate cancer before and during the cytotoxic therapy. Detection will be performed by the method of immunomagnetic separation and RNA isolation from this fraction. Then we will perform the cultivation of the detected cells including the cultivation in milieu of cytotoxic agents. In the next stage the genetic profile of the detected cells will be assessed (a panel of 24 genes) and a similar profile will be evaluated also in histological specimen of a primary tumor. Analysis results will be correlated to the clinical course of disease and the prognostic factors of treatment effect on CTC count decline and patients\u2018 survival will be determined."@en . . . "2014-03-21+01:00"^^ . "2015-01-22+01:00"^^ . "Detection of circulating tumor cells and gene expression assessment in castration resistant prostate cancer as part of systemic therapy individualization"@en . .