"The main aim of this project is to search for new and convenient therapeutics that will be more effective in therapy of pesticide poisoning (pesticides are commonly used organophosphate inhibitors of cholinesterases). New structures will be determined by using highly sophistic methodology: molecular design. Predicted structures and their analogues will be synthesized. Their therapeutic potency will be tested in in vitro studies. The acetylcholinesterase reactivators with the highest reactivation potency will be chosen into in vivo tests. During this development process other important data such as LD50 of newly synthesized therapeutics, biochemical studies and pharmacokinetics studies (distribution of newly prepared therapeutics and their ability to penetrate through the blood-brain barrier into central nervous system) will be also replenished. If some effective candidates will be found, subsequent cooperation with foreign institutes is presumed."@en . . . "Hlavn\u00EDm p\u0159edm\u011Btem \u0159e\u0161en\u00ED tohoto projektu je hled\u00E1n\u00ED nov\u00E9ho vhodn\u00E9ho l\u00E9\u010Diva, kter\u00E9 by bylo \u00FA\u010Dinn\u011Bj\u0161\u00ED v terapii intoxikac\u00ED zp\u016Fsoben\u00FDch pesticidy (v praxi \u0161iroce u\u017E\u00EDvan\u00E9 inhibitory cholinesteras). V r\u00E1mci projektu dojde k synt\u00E9ze nov\u00FDch reaktiv\u00E1tor\u016F AChE podpo\u0159en\u00E9 molekul\u00E1rn\u00EDm modelov\u00E1n\u00EDm. U nov\u011B syntetizovan\u00FDch chemick\u00FDch individu\u00ED prob\u011Bhne z\u00E1kladn\u00ED in vitro screening, kter\u00FD ur\u010D\u00ED nej\u00FA\u010Dinn\u011Bj\u0161\u00ED kandid\u00E1ty do n\u00E1sledn\u00FDch in vivo test\u016F (ov\u011B\u0159en\u00ED \u00FA\u010Dinnosti po pod\u00E1n\u00ED). V pr\u016Fb\u011Bhu \u0159e\u0161en\u00ED budou dopl\u0148ov\u00E1na i dal\u0161\u00ED data - ur\u010Deny LD50 vybran\u00FDch reaktiv\u00E1tor\u016F, prob\u011Bhnou biochemick\u00E9 screeningy \u010Di farmakokinetick\u00E9 studie, kter\u00E9 pop\u00ED\u0161\u00ED distribuci l\u00E9\u010Diva v organismu a jeho schopnost prostoupit p\u0159es hematoencefalickou bari\u00E9ru." . "1"^^ . . "P\u0159\u00EDprava a hodnocen\u00ED \u00FA\u010Dinnosti nov\u00FDch terapeutik proti otrav\u00E1m pesticidy" . . . " acetylcholinesterase" . "2014-12-31+01:00"^^ . . . "0"^^ . . . "2015-01-22+01:00"^^ . . . "2013-08-09+02:00"^^ . . . . "pesticides" . . "10"^^ . "Preparation and biological evaluation of new therapeutics against to pesticides"@en . "10"^^ . . . "NT12062" . "pesticides; acetylcholinesterase; therapeutics; reactivator; oxime"@en . "2011-06-01+02:00"^^ . " reactivator" . "http://www.isvav.cz/projectDetail.do?rowId=NT12062"^^ . " therapeutics" . "0"^^ .