"Projekt prok\u00E1zal v\u00FDznam p\u0159id\u00E1n\u00ED pomocn\u00FDch epitop\u016F i mikroprost\u0159ed\u00ED n\u00E1doru v \u00FA\u010Dinnost DNA vakc\u00EDn."@cs . . "DNA vaccines; helper epitope; SOX2; NANOG; xenogeneic genes; localization signals"@en . . . "2014-09-02+02:00"^^ . . " NANOG" . " SOX2" . "Development of vaccines against markers of cancer stem cells"@en . . . "NT11541" . "Development of DNA vaccines against transcription factors SOX2 and NANOG that are necessary for self-renewal of cancer stem cells in various human tumors. Enhancement of immunogenicity of these vaccines by the addition of cell localization signals and sequences from the tetanus toxin that encode strong helper epitopes activating Th cells. Analysis of the effect of xenogeneic genes on vaccine efficacy. Reduction of potential oncogenicity of SOX2 and NANOG by the mutagenesis of nuclear localization signals and by the removal of their initiation codons in fusion genes. Detection of immune responses induced with the constructed DNA vaccines by in vitro tests and by observation of an anti-tumor effect of the vaccines in mouse tumor models using embryonal carcinoma cell lines."@en . . "0"^^ . "http://www.isvav.cz/projectDetail.do?rowId=NT11541"^^ . "2013-12-31+01:00"^^ . . . . . "DNA vaccines" . "1"^^ . " xenogeneic genes" . "The project demonstrated the significance of helper epitopes and tumor microenvironment in the mechanism of action of DNA vaccines."@en . "2"^^ . . . "0"^^ . "2013-03-26+01:00"^^ . "2"^^ . "V\u00FDvoj vakc\u00EDn nam\u00ED\u0159en\u00FDch proti znak\u016Fm n\u00E1dorov\u00FDch kmenov\u00FDch bun\u011Bk" . . . . "2010-09-01+02:00"^^ . " helper epitope" . "V\u00FDvoj DNA vakc\u00EDn proti transkrip\u010Dn\u00EDm faktor\u016Fm SOX2 a NANOG, kter\u00E9 jsou nezbytn\u00E9 pro sebeobnovu n\u00E1dorov\u00FDch kmenov\u00FDch bun\u011Bk u r\u016Fzn\u00FDch typ\u016F lidsk\u00FDch n\u00E1dor\u016F. Zv\u00FD\u0161en\u00ED imunogennosti t\u011Bchto vakc\u00EDn p\u0159id\u00E1n\u00EDm bun\u011B\u010Dn\u00FDch lokaliza\u010Dn\u00EDch sign\u00E1l\u016F a sekvenc\u00ED z genu tetanov\u00E9ho toxinu k\u00F3duj\u00EDc\u00EDch siln\u00E9 pomocn\u00E9 epitopy aktivuj\u00EDc\u00ED bu\u0148ky Th. Anal\u00FDza vlivu xenogenn\u00EDch gen\u016F na imunogennost vakc\u00EDn. Sn\u00ED\u017Een\u00ED potenci\u00E1ln\u00ED onkogennosti SOX2 a NANOG mutagenez\u00ED jadern\u00FDch lokaliza\u010Dn\u00EDch sign\u00E1l\u016F a odstran\u011Bn\u00EDm jejich inicia\u010Dn\u00EDch kodon\u016F u f\u00FAzn\u00EDch gen\u016F. Stanoven\u00ED imunitn\u00EDch reakc\u00ED vyvolan\u00FDch p\u0159ipraven\u00FDmi DNA vakc\u00EDnami testy in vitro a sledov\u00E1n\u00EDm protin\u00E1dorov\u00E9ho \u00FA\u010Dinku na my\u0161\u00EDch n\u00E1dorov\u00FDch modelech s pou\u017Eit\u00EDm bun\u011B\u010Dn\u00FDch lini\u00ED z\u00EDskan\u00FDch z embryon\u00E1ln\u00EDch karcinom\u016F." . . .