. "2013-03-26+01:00"^^ . . "Type 1 diabetes arises from autoimmune insulitis that is initiated in genetically predisposed individuals by yet unknown environmental triggers. Enteroviruses belong to the suspected agents. The project utilizes samples from the Norwegian newborn %22MIDIA%22 cohort that consists of children selected at birth based on their highest HLA-encoded genetic risk of type 1 diabetes. We propose a nested case-control study where cases are children with islet autoimmunity (two or more autoantibodies), and controls are matched 2:1 by place and time of birth. The tested exposure will be the occurrence of enterovirus and parechovirus in blood and stool; effort will be made towards complex genetic characteristics of the virus present in blood, including regions outside the VP1, utilizing the synchronous stool samples. The expected outcomes are improvements in the environmental factors in pathogenesis of type 1 diabetes."@en . "NT11465" . . "2014-12-31+01:00"^^ . "4"^^ . " picornavirus" . "0"^^ . "4"^^ . "1"^^ . . "islet autoimmunity; picornavirus; enterovirus; parechovirus; type 1 diabetes pathogenesis; nested case-control study"@en . . "0"^^ . "2015-01-22+01:00"^^ . . " enterovirus" . . "Diabetes 1. typu vznik\u00E1 n\u00E1sledkem autoimunitn\u00ED inzulit\u00EDdy, kter\u00E1 je iniciov\u00E1na u geneticky predisponovan\u00FDch jedinc\u016F dosud nezn\u00E1m\u00FDmi vlivy prost\u0159ed\u00ED. Za podez\u0159el\u00E9 jsou pova\u017Eov\u00E1ny zejm\u00E9na enteroviry z rodiny pikornavir\u016F. Projekt vyu\u017E\u00EDv\u00E1 vzork\u016F z norsk\u00E9 novorozeneck\u00E9 kohorty 'MIDIA', kterou tvo\u0159\u00ED jedinci vybran\u00ED na z\u00E1klad\u011B nejvy\u0161\u0161\u00EDho genetick\u00E9ho rizika diabetu 1. typu. Podstatou je proveden\u00ED vno\u0159en\u00E9 ('nested') studie p\u0159\u00EDpad\u016F a kontrol, kde p\u0159\u00EDpady budou prediabetick\u00E9 d\u011Bti (dv\u011B a v\u00EDce autoprotil\u00E1tek) a kontroly budou dva jedinci k p\u0159\u00EDpadu struktur\u00E1ln\u011B vyrovnan\u00ED ('matched') podle m\u00EDsta a doby narozen\u00ED. Vy\u0161et\u0159ovanou expozic\u00ED bude v\u00FDskyt enteroviru a parechoviru v krvi a stolici, p\u0159i\u010Dem\u017E vyvineme \u00FAsil\u00ED o jeho charakterizaci zejm\u00E9na mimo oblasti VP1, v\u010Detn\u011B pokusu o celogenomovou sekvenaci viru p\u0159\u00EDmo z extrahovan\u00E9 RNA bez nutnosti jeho izolace na bun\u011B\u010Dn\u00E9 kultu\u0159e. O\u010Dek\u00E1van\u00FDm v\u00FDsledkem je zlep\u0161en\u00ED znalost\u00ED o negenetick\u00FDch vlivech v patogenezi diabetu 1. typu, a to publikovan\u00E9 v mezin\u00E1rodn\u00EDm odborn\u00E9m tisku." . "Selected picornaviruses in the pathogenesis of islet autoimmunity and type 1 diabetes"@en . . . . "islet autoimmunity" . " parechovirus" . . . "http://www.isvav.cz/projectDetail.do?rowId=NT11465"^^ . . "Vybran\u00E9 pikornaviry v patogenezi ostr\u016Fvkov\u00E9 autoimunity a diabetu 1. typu" . . " type 1 diabetes pathogenesis" . "2010-09-01+02:00"^^ . . . . .