"http://www.isvav.cz/projectDetail.do?rowId=NS9652"^^ . . "0"^^ . "1"^^ . "CLL; allelic exclusion; allelic inclusion; RNA microarrays; signaling pathways"@en . . "1"^^ . "Allelic exclusion is a unique phenomenon, ensuring antibody monospecificity of B-lymphocytes. The process is strictly controlled and aberrant B-lymphocytes, expressing two IgVH alleles, are eliminated. Chronic lymphocytic leukemia (CLL) is a clonal disorder of B-lymphocytes. In 5-8% of cases, expression of two productive IgVH rearrangements have been observed, a process referred to as allelic inclusion. CLL exhibiting allelic inclusion represents a unique model to study the process of allelic exclusion. By microarray RNA expression profiling of clonal CLL cells with allelic exclusion, allelic inclusion and peripheral B-lymphocytes from healthy donors it is possible to identify genes that might play a role in the process of allelic exclusion. By employing gene modulating techniques and tissue cultures it is possible to test the biological effects of candidate genes as identified by RNA microarrays in vitro and delineate signaling pathways involved in the process of allelic exclusion of B- lymphocytes."@en . . "1"^^ . "1"^^ . . "Identifikace nov\u00FDch molekul\u00E1rn\u00EDch sign\u00E1ln\u00EDch drah v procesu alelick\u00E9 exkluze; chronick\u00E1 lymfocyt\u00E1rn\u00ED leuk\u00E9mie jako modelov\u00FD syt\u00E9m" . " RNA microarrays" . "2009-01-01+01:00"^^ . . . " allelic inclusion" . "CLL" . "2011-08-08+02:00"^^ . "2011-12-31+01:00"^^ . . "NS9652" . . . . . . "Alelick\u00E1 exkluze je unik\u00E1tn\u00EDm fenom\u00E9nem, kter\u00FD zaji\u0161\u0165uje protil\u00E1tkovou monospecifitu B-lymfocyt\u016F. Alelick\u00E1 exkluze je velmi p\u0159\u00EDsn\u011B kontrolov\u00E1na a aberantn\u00ED B-lymfocyty jsou v naprost\u00E9 v\u011Bt\u0161in\u011B p\u0159\u00EDpad\u016F eliminov\u00E1ny. Chronick\u00E1 lymfocyt\u00E1rn\u00ED leuk\u00E9mie (CLL) je klon\u00E1ln\u00ED onemocn\u011Bn\u00ED B-lymfocyt\u016F. U 5-8 % p\u0159\u00EDpad\u016F byl pozorov\u00E1n unik\u00E1tn\u00ED jev alelick\u00E9 inkluze, neboli p\u0159\u00EDtomnosti dvou produktivn\u00EDch IgVH p\u0159estaveb v jedn\u00E9 bu\u0148ce. CLL s fenom\u00E9nem alelick\u00E9 inkluze p\u0159edstavuje unik\u00E1tn\u00ED model ke studiu procesu alelick\u00E9 exkluze. Srovn\u00E1n\u00EDm RNA expresn\u00EDch profil\u016F klon\u00E1ln\u00EDch CLL bun\u011Bk s alelickou exkluz\u00ED, alelickou inkluz\u00ED a zdrav\u00FDch B lymfocyt\u016F je mo\u017En\u00E9 identifikovat diferenci\u00E1ln\u011B exprimovan\u00E9 geny, kter\u00FDch produkty mohou hr\u00E1t roli v procesu alelick\u00E9 exkluze. Pomoc\u00ED technik genov\u00E9ho in\u017Een\u00FDrstv\u00ED lze in vitro otestovat funkci kandid\u00E1tn\u00EDch gen\u016F identifikovan\u00FDch pomoc\u00ED RNA microarrays a pokusit se osv\u011Btlit molekul\u00E1rn\u00ED sign\u00E1ln\u00ED dr\u00E1hy, d\u016Fle\u017Eit\u00E9 pro unik\u00E1tn\u00ED proces alelick\u00E9 exkluze B-lymfocyt\u016F." . . "The authors did not elucidate the moplecular mechanism of allelic exclusion in chronic lymphocytic leukemia."@en . . "Identification of novel molecular pathways involved in the process of B-cell allelic exclusion; chronic lymphocytic leukemia as the model system"@en . . " allelic exclusion" . "2013-10-31+01:00"^^ . . . . "Auto\u0159i nepotvrdili anotovanou hypot\u00E9zu o mechanizmu alelick\u00E9 exkluse u pacient\u016F s chronickou lymfocyt\u00E1rn\u00ED leukemi\u00ED."@cs .