. . . "2009-01-01+01:00"^^ . "Annotation I: Peripheral blood and synovial fluid will be obtained from patients with rheumatoid arthritis and osteoarthritis. Synovial membrane and hyaline cartilage will be obtained from the registry of samples of the Institute of Rheumatology. Annotation II: FABP4 will be analyzed in serum, synovial fluid, synovial membrane and hyaline cartilage. Methods of immunohistochemistry, commercially available ELISA kit, Western blot and gene expression will be used for the analysis. An extracellular role of FABP4 will be studied with stimulation experiments on cell lines of monocytes, synovial fibroblasts and chondrocytes. An impact of FABP4 on the expression of genes participating in the pathogenesis of rheumatic diseases will be studied. Annotation III: An intracellular role of increased expression of FABP4 by the cells of articular compartment will be evaluated by gene interference. A relationship of FABP4 to an inflammatory state, activity and severity of the disease will be evaluated."@en . "Prok\u00E1z\u00E1no zv\u00FD\u0161en\u00E9 mno\u017Estv\u00ED FABP4 proteinu v synovi\u00E1ln\u00ED membr\u00E1n\u011B, synovi\u00E1ln\u00EDn tekutin\u011B a cirkulaci u pacient\u016F s RA. Zv\u00FD\u0161en\u00E1 exprese FABP4 u nemocn\u00FDch s RA a jeho asociace s lipidov\u00FDm profilem poukazuje na jeho v\u00FDznam v regulaci metabolismu."@cs . "fatty acid-binding protein; rheumatoid arthritis; osteoarthritis"@en . . . "fatty acid-binding protein" . . . . "Anotace I: Perifern\u00ED krev a synovi\u00E1ln\u00ED tekutina bude z\u00EDsk\u00E1na od pacient\u016F s revmatoidn\u00ED artritidou a osteoartr\u00F3zou. Synovi\u00E1ln\u00ED membr\u00E1na a hyalinn\u00ED chrupavka budou z\u00EDsk\u00E1ny z registru vzork\u016F Revmatologick\u00E9ho \u00FAstavu. Monocyty budou izolov\u00E1ny z krve a synovi\u00E1ln\u00ED tekutiny, synovi\u00E1ln\u00ED fibroblasty a chondrocyty budou izolov\u00E1ny z p\u0159\u00EDslu\u0161n\u00FDch tk\u00E1n\u00ED. Anotace II: Protein FABP4 bude analyzov\u00E1n v s\u00E9ru, synovi\u00E1ln\u00ED tekutin\u011B, synovi\u00E1ln\u00ED membr\u00E1n\u011B a hyalinn\u00ED chrupavce. K anal\u00FDze budou pou\u017Eity metody imunohistochemick\u00E9, komer\u010Dn\u011B dostupn\u00FD ELISA kit, Western blot a genov\u00E1 exprese. Extracelul\u00E1rn\u00ED \u00FAloha FABP4 bude studov\u00E1na pomoc\u00ED stimula\u010Dn\u00EDch experiment\u016F na lini\u00EDch monocyt\u016F, synovi\u00E1ln\u00EDch fibroblast\u016F a chondrocyt\u016F. Studov\u00E1n bude vliv FABP4 na expresi gen\u016F pod\u00EDlej\u00EDc\u00EDch se na patogenezi revmatick\u00FDch onemocn\u011Bn\u00ED. Anotace III: Intracelul\u00E1rn\u00ED \u00FAloha zv\u00FD\u0161en\u011B exprimovan\u00E9ho FABP4 bu\u0148kami kloubn\u00EDho kompartmentu bude hodnocena pomoc\u00ED genov\u00E9 interference. Bude hodnocen i vztah FABP4 k z\u00E1n\u011Btliv\u00E9mu stavu, aktivit\u011B a z\u00E1va\u017Enosti onemocn\u011Bn\u00ED" . "1"^^ . "0"^^ . . "Contribution of adipocyte/macrophage fatty acid-binding protein (FABP)-4 to the pathogenesis of rheumatoid arthritis and osteoarthritis"@en . "1"^^ . "http://www.isvav.cz/projectDetail.do?rowId=NS10620"^^ . " rheumatoid arthritis" . "1"^^ . . . . . "0"^^ . . "2011-08-08+02:00"^^ . "2011-12-31+01:00"^^ . . "Role proteinu v\u00E1zaj\u00EDc\u00EDho mastn\u00E9 kyseliny (FABP4) v patogenezi revmatoidn\u00ED artritidy a osteoartr\u00F3zy" . . "NS10620" . "It was shown there is increased FABP4 in rheumatoid synovial tissue, synovial fluid and blood circiulation of patient with RA. Increased amount of FABP4 and its possitive correlation with lipid profile suggest that it may be related to metabolic changes"@en . . . . "2013-10-31+01:00"^^ .