. "Male infertility" . "2013-10-31+01:00"^^ . "Exprese gen\u016F a protein\u016F, kter\u00E9 maj\u00ED \u00FAlohu v lidsk\u00E9 reprodukci - biomarkery po\u0161kozen\u00ED" . . . . . "0"^^ . "http://www.isvav.cz/projectDetail.do?rowId=NS10009"^^ . "Posledn\u00ED studie ukazuj\u00ED na zvy\u0161uj\u00EDc\u00ED se n\u00E1r\u016Fst defekt\u016F mu\u017Esk\u00FDch reproduk\u010Dn\u00EDch org\u00E1n\u016F a sni\u017Eov\u00E1n\u00ED kvality spermatu - tyto faktory byly pops\u00E1ny jako %22Testicular dysgenesis syndrom%22 TDS. Zjistit expresi gen\u016F k\u00F3duj\u00EDc\u00EDch vybran\u00E9 proteiny (akrosom\u00E1ln\u00ED-, androgenn\u00ED- %22heat shock%22-, ubiquitin- apoptotick\u00E9-) v testes neplodn\u00FDch mu\u017E\u016F pomoc\u00ED semikvantitativn\u00ED RT-PCR. Zjistit rozd\u00EDly v detekci/lokalizaci vybran\u00FDch protein\u016F (povrchov\u00FDch a intra-akrosom\u00E1ln\u00EDch, cytoskelet\u00E1ln\u00EDch a %22heat shock%22 protein\u016F) na spermi\u00EDch a v semenn\u00E9 plasm\u011B u mu\u017E\u016F s norm\u00E1ln\u00EDm a patologick\u00FDm spermiogramem. Zjistit vztah mezi expres\u00ED gen\u016F v testes a detekc\u00ED protein\u016F na spermi\u00EDch, ur\u010Dit biomarkery po\u0161kozen\u00ED. Vyvinout nov\u00E9 metody a n\u00E1stroje pro objektivn\u00ED a citlivou diagnostiku mu\u017Esk\u00E9 neplodnosti." . . " Intra-acrosomal proteins" . "1"^^ . "2011-12-31+01:00"^^ . "1"^^ . "Monoklon\u00E1ln\u00ED protil\u00E1tky lze vyu\u017E\u00EDt jako n\u00E1stroj k detekci a lokalizaci zm\u011Bn protein\u016F spermie a semenn\u00E9 plasmy.Identifikace protein\u016F a ur\u010Den\u00ED enzymatick\u00E9 aktivity v ejakul\u00E1tech pacient\u016F m\u016F\u017Ee objasnit poruchy fertilizace u mu\u017E\u016F s patologick\u00FDm spermiogramem"@cs . . " Damage biomarkers" . "2011-08-08+02:00"^^ . . . . . . "5"^^ . "Male infertility; Gene expression; Cell surface sperm proteins; Intra-acrosomal proteins; Seminal plasma; Damage biomarkers; Human environment"@en . "5"^^ . "Expression of genes and proteins playing a role in the human reproduction - damage biomarkers"@en . "Monoclonal antibodies have confirmed capacity to be used as tools for detection and localization of changes in sperm proteins and seminal plasma related to patology."@en . . . . . " Cell surface sperm proteins" . . "NS10009" . . "2009-01-01+01:00"^^ . " Gene expression" . . "Recent studies show an increase in the development of defects of reproductive organs, and a decrease in the sperm quality - these are symptomps of one underlying entity - the dysgenesis syndrome. To examine the expression level of acrosome-, androgen-, heat shock-, ubiquitin- and apoptosis-related genes by semiquantitative RT-PCR in the samples of testicular biopsies of infertile men. To find the differences in localization of sperm, cytoskeletal and heat shock proteins in the sperm and seminal plasma between healthy and subfertile men by specific antibodies. To assess the relationship between gene expression in testes and sperm and seminal plasma proteins detection and to develop new methods and tools for objective and sensitive diagnosis of male infertility."@en . " Seminal plasma" . .