"DNA vaccine" . . . "NR9246" . . "Sestrojen\u00ED DNA vakc\u00EDny se zv\u00FD\u0161enou imunogennost\u00ED v\u016F\u010Di onkoprotein\u016F E6, E7."@cs . "V\u00FDvoj protin\u00E1dorov\u00FDch DNA vakc\u00EDn zalo\u017Een\u00FDch na f\u00FAzi imunogenu s beta-glukuronid\u00E1zou E. coli a zv\u00FD\u0161en\u00ED jejich \u00FA\u010Dinku stimulac\u00ED nespecifick\u00E9 imunity" . . . " Toll-like receptor" . . . " tumor escape" . " HPV16" . . " fusion gene" . "http://www.isvav.cz/projectDetail.do?rowId=NR9246"^^ . "DNA vaccine; fusion gene; immunostimulatory drug; tumor escape; antigen presentation; Toll-like receptor; HPV16; CML"@en . . . "2007-01-01+01:00"^^ . . " immunostimulatory drug" . . . . "Construction of DNA vaccine with enhanced immunogenicity against E6 and E7 oncoproteins."@en . "2009-12-31+01:00"^^ . "Zv\u00FD\u0161en\u00ED \u00FA\u010Dinnosti terapeutick\u00FDch protin\u00E1dorov\u00FDch vakc\u00EDn zalo\u017Een\u00FDch na f\u00FAzi n\u00E1dorov\u00E9ho antigenu s beta-glukuronid\u00E1zou E. coli. Modifikace gen\u016F sm\u011B\u0159uj\u00EDc\u00ED ke zv\u00FD\u0161en\u00ED produkce imunogen\u016F, kter\u00FDmi budou onkoproteiny E7 a E6 HPV16 a f\u00FAzn\u00ED onkoprotein Bcr-Abl. Stanoven\u00ED mechanism\u016F uplat\u0148uj\u00EDc\u00EDch se p\u0159i indukci imunitn\u00EDch reakc\u00ED po aplikaci f\u00FAzn\u00EDch gen\u016F intraderm\u00E1ln\u011B genovou pistol\u00ED nebo intramuskul\u00E1rn\u011B injikac\u00ED. Testov\u00E1n\u00ED \u00FA\u010Dink\u016F imunizac\u00ED na my\u0161\u00EDch n\u00E1dorov\u00FDch modelech. Pos\u00EDlen\u00ED protin\u00E1dorov\u00E9ho \u00FA\u010Dinku DNA vakc\u00EDn kombinac\u00ED jednotliv\u00FDch f\u00FAzn\u00EDch gen\u016F a d\u00E1le kombinac\u00ED s l\u00E1tkami stimuluj\u00EDc\u00EDmi vrozenou imunitu, a to zejm\u00E9na p\u0159es receptory TLR (Toll-like receptors). Sledov\u00E1n\u00ED mo\u017Enosti odstran\u011Bn\u00ED n\u00E1dorov\u00FDch bun\u011Bk vyzna\u010Duj\u00EDc\u00EDch se r\u016Fzn\u00FDmi mechanismy \u00FAniku z hostitelsk\u00E9 imunity, zejm\u00E9na poklesem tvorby molekul MHC-I a mutovan\u00FDm imunodominantn\u00EDm epitopem n\u00E1dorov\u00E9ho antigenu" . . . "0"^^ . . . "4"^^ . "0"^^ . "2009-12-18+01:00"^^ . "2011-04-27+02:00"^^ . "4"^^ . "1"^^ . " antigen presentation" . "Enhancement of efficacy of therapeutic anti-tumor vaccines based on fusion of a tumor antigen with E. coli beta-glucuronidase. Increase of production of immunogens (oncoproteins E7 and E6 of HPV16 and fusion oncoprotein Bcr-Abl) by modification of genes. Determination of mechanisms involved in induction of immune reactions after administration of fusion genes intradermally by a gene gun or intramuscularly by injection. Examination of immunization effects in mouse tumor models. Enhancement of anti-tumor efficacy of DNA vaccines by combination of single fusion genes and by combination with drugs stimulating innate immunity, particularly via Toll-like receptors. Elimination of tumor cells with different mechanisms of escape from the host immunity, especially down-regulation of MHC class I surface expression and mutation of the immunodominant epitope of a tumor antigen.."@en . "Development of anti-tumor DNA vaccines based on fusion of immunogen with E. coli beta-glucuronidase and enhancement of their effect by stimulation of non-specific immunity"@en .