"1"^^ . "Projekt vych\u00E1z\u00ED z na\u0161\u00ED dosavadn\u00ED v\u00FDzkumn\u00E9 pr\u00E1ce, ve kter\u00E9 jsme tlumen\u00EDm genov\u00E9 exprese pomoc\u00ED siRNA analyzovali mo\u017Enost ovlivn\u011Bn\u00ED funkce gen\u016F, jejich\u017E zm\u011Bny aktivity byly spojeny s r\u016Fzn\u00FDmi onko-hematologick\u00FDmi procesy. Na rozd\u00EDl od pou\u017Eit\u00ED siRNA je tento projekt zam\u011B\u0159en na anal\u00FDzu miRNA, kter\u00E1 dovoluje i sledov\u00E1n\u00ED dereprese genov\u00E9 funkce blokov\u00E1n\u00EDm endogenn\u00EDch miRNA. Sou\u010D\u00E1st\u00ED projektu je 1) profilov\u00E1n\u00ED miRNA \u010Dipovou technikou na bu\u0148k\u00E1ch bun\u011B\u010Dn\u00FDch lini\u00ED a r\u016Fzn\u00FDch bun\u011B\u010Dn\u00FDch typech izolovan\u00FDch z perifern\u00ED krve zdrav\u00FDch osob a 2) sledov\u00E1n\u00ED zm\u011Bn genov\u00E9 aktivity po blokaci vybran\u00FDch miRNA s pou\u017Eit\u00EDm anti-miRNA a to jak na \u00FArovni transkripce tak translace. V pr\u00E1ci budou pou\u017Eity bun\u011B\u010Dn\u00E9 linie K562, KU182, MOLM7, HL60 a HEL a celkov\u00E9 leukocyty z\u00EDskan\u00E9 z perifern\u00ED krve zdrav\u00FDch dobrovoln\u00EDk\u016F oboj\u00EDho pohlav\u00ED a r\u016Fzn\u00FDch v\u011Bkov\u00FDch kategori\u00ED, p\u0159\u00EDpadn\u011B monocyty a granulocyty a izolovan\u00E9 bun\u011B\u010Dn\u00E9 typy CD34+, CD14+, CD3+. Sledov\u00E1ny budou v hemopoetick\u00FDch bu\u0148k\u00E1ch exprimovan\u00E9 miRNA: miR15, miR16, miR142, miR155 a miR223." . . "2011-04-27+02:00"^^ . "0"^^ . . "miRNA" . "0"^^ . "2009-12-31+01:00"^^ . "Endogeneous post-transcriptional regulation of gene function by miRNA"@en . "The project is based on our previous research study, in which using siRNA-mediated gene silencing we analyzed possible function modulation of the genes whose dysregulation was associated with various onco-hematological processes. In contrast to siRNAs, this project is focused on miRNA analyses that also allow to observe de-repression of gene function using miRNA inhibitors. The project includes: 1) miRNA expression profiling in cell lines and in peripheral blood cells of healthy persons using microarrays; 2) analyses of gene activity changes after inhibition of selected miRNAs using Anti-miRNAs at both transcriptional and translational level. In this study we will use cell lines K562, KU812, MOLM7, HL60, HEL and total peripheral blood leukocytes obtained from healthy donors, eventually monocytes and granulocytes or cell subtypes CD34+, CD14+, CD3+ will be used. Expression of miR15, miR16, miR142, miR155 and miR223 will be monitored in hematopoiteic cells."@en . . . "http://www.isvav.cz/projectDetail.do?rowId=NR9236"^^ . . . "2007-01-01+01:00"^^ . "NR9236" . "ENDOGENN\u00CD POST-TRANSKRIP\u010CN\u00CD REGULACE GENOV\u00C9 FUNKCE PROST\u0158EDNICTV\u00CDM miRNA" . . " expression profiling" . . . . . . . "2009-12-18+01:00"^^ . "miRNA are important regulatory factors of hematopoeisis and represent relevant markers of clonal hematological disorders such as polycytemia vera."@en . "miRNA jsou d\u016Fle\u017Eit\u00FDmi regula\u010Dn\u00EDmi faktory hematopo\u00E9zy a \u017Ee jsou v\u00FDznamn\u00FDmi markery klon\u00E1ln\u00EDch hematologick\u00FDch onemocn\u011Bn\u00ED typu polycytemia vera."@cs . . " protein expression" . . . "7"^^ . "7"^^ . . . . "miRNA; anti-miRNA; expression profiling; protein expression; miRNA target genes"@en . . " anti-miRNA" .