"rheumatoid arthritis; S100A4; apoptosis; pro-inflammatory cytokines; matrix degrading enzymes; gene expression; siRNA"@en . . "Perifern\u00ED krevn\u00ED monocyty budou z\u00EDsk\u00E1ny sto\u010Den\u00EDm krve po krevn\u00EDm odb\u011Bru a synovi\u00E1ln\u00ED fibroblasty po mechanick\u00E9 a enzymatick\u00E9 \u00FAprav\u011B synovi\u00E1ln\u00ED tk\u00E1n\u011B z\u00EDskan\u00E9 b\u011Bhem transplantace kloubu. Extracelul\u00E1rn\u00ED p\u016Fsoben\u00ED bude studov\u00E1no pomoc\u00ED stimula\u010Dn\u00EDch experiment\u016F s vyu\u017Eit\u00EDm inhibice receptoru RAGE a intracelul\u00E1rn\u00ED \u00FAloha pomoc\u00ED utlumen\u00ED genov\u00E9 exprese za pou\u017Eit\u00ED mal\u00E9ho inhibitoru (si) RNA s vyu\u017Eit\u00EDm nukleofek\u010Dn\u00EDho p\u0159\u00EDstroje Amaxa. Genov\u00E1 exprese apoptotick\u00FDch molekul (bax, bcl rodiny, XIAP, smac), proz\u00E1n\u011Btliv\u00FDch cytokin\u016F (TNF, IL-1, IL-6 a MCP-1) a matrix degraduj\u00EDc\u00EDch enzym\u016F (MMP-1, MMP-3 a MMP-13) bude analyzov\u00E1na pomoc\u00ED real-time PCR. Synt\u00E9za protein\u016F bude potvrzena pomoc\u00ED metod ELISA a Western-blot. Aktivace dr\u00E1hy vedouc\u00ED k programovan\u00E9 smrti bun\u011Bk bude analyzov\u00E1na pomoc\u00ED fluorimetrick\u00E9 eseje m\u011B\u0159\u00EDc\u00ED aktivitu kasp\u00E1zy-3." . . "Role of S100A4 (metastasin) in the pathogenesis of rheumatoid arthritis"@en . "http://www.isvav.cz/projectDetail.do?rowId=NR9082"^^ . . . "NR9082" . . " matrix degrading enzymes" . " pro-inflammatory cytokines" . " apoptosis" . "2006-01-01+01:00"^^ . . " S100A4" . . "4"^^ . "2011-04-27+02:00"^^ . "Increased S100A4 protein was demonstrated in plasma, synovial fluid and hyperplastic synovial membrane of pts with RA and also relationsh S100A4 and pathogenezis of RA as well as a positive association between the levels of S100A4 and disease activity"@en . "4"^^ . "1"^^ . . "1"^^ . . . . "Bylo prok\u00E1z\u00E1no zv\u00FD\u0161en\u00E9 mno\u017Estv\u00ED S100A4 proteinu v plazm\u011B, synovi\u00E1ln\u00ED tekutin\u011B a hyperplastick\u00E9 synovi\u00E1ln\u00ED membr\u00E1n\u011B pacient\u016F s RA. Byl zji\u0161t\u011Bn vztah S100A4 k patogenezi RA a pozitivn\u00ED asociace mezi hladinami S100A4 s klinickou aktivitou nemoci"@cs . . . . "rheumatoid arthritis" . "V\u00FDznam S100A4 proteinu (metastasin) v patogenezi revmatoidn\u00ED artritidy" . " gene expression" . "0"^^ . . "2009-12-18+01:00"^^ . "Peripheral blood monocytes will be obtained after the blood withdrawal and synovial fibroblasts after the mechanical and enzymatical modifications of the synovial tissue obtained during the joint surgery. Extracellular roles will be studied by stimulatory experiments with an additional inhibition of the RAGE and intracellular roles by means of gene silencing using small interfering (si) RNA. Nucleofector device Amaxa will be used for cell transfection. Gene expression of apoptotic molecules (bax, bcl-family, XIAP, smac), pro-inflammatory cytokines (TNF, IL-1, IL-6 and MCP-1) and matrix degrading enzymes (MMP-1, MMP-3 and MMP-13) will be analyzed using real-time PCR. The protein synthesis will be confirmed by means of ELISA and Western-blot. Activation of the programmed cell death pathway will be measured using fluorimetric Caspase-3 activity assay."@en . . . . . "2009-12-31+01:00"^^ .