" MMP-2 polymorphismus" . . "2006-01-01+01:00"^^ . . "muliple sclerosis" . " MMP-2" . "2011-04-27+02:00"^^ . "8"^^ . . "8"^^ . . . . . . . "2009-12-18+01:00"^^ . "Lower frequency of T -1562 alel of MMP-9 gene was found in remitting multiple sclerosis patients in comparison with healthy controls. Increased serum MMP-2 levels and decreased TIMP-2 levels correlated with disability in multiple sclerosis."@en . "http://www.isvav.cz/projectDetail.do?rowId=NR8832"^^ . . " genotypization" . "0"^^ . "Matrixmetalloprotein\u00E1zy v imunopatogeneze roztrou\u0161en\u00E9 skler\u00F3zy" . . "1"^^ . "The aim of this study is to analyse the activity of MMP-2, MMP-9 and their inhibitors in MS and to assess their relationship to the disease activity, its form, and any correlation with the clinical disability and therapeutic influences and further to to examine the possible association between genetic polymorphism in candidate genes with multiple sclerosis.The study is to include 80 MS patients and 50 healthy controls. MMP-2, MMP-9, TIMP-2 TIMP-1 serum levels will be quantified by ELISA. Serum levels will beassessed at the beginning of the study, during exacerbations and at the end of the study. Genetic analysis will be performed by standard molecular biology practice.DNA and RNA will be isolated from blood.The PCR method,restrict and heteroduplex analysis are to be employed. MMPs are a promising biological marker that enables us to assess the activity of the disease and to monitor the effects of treatment."@en . "C\u00EDlem pr\u00E1ce je anal\u00FDza aktivity MMP-2,MMP-9 a jejich inhibitor\u016F a hodnotit vztah k aktivit\u011B, form\u011B choroby, t\u00ED\u017Ei klinick\u00E9ho posti\u017Een\u00ED a ovlivn\u011Bn\u00ED l\u00E9\u010Dbou.Ur\u010Dit asocia\u010Dn\u00ED vztah genetick\u00FDch polymorfism\u016F lokalizovan\u00FDch v kandid\u00E1tn\u00EDch genech s MS. Soubor bude tvo\u0159en asi 80-ti pacienty s roztrou\u0161enou skler\u00F3zou dle Mc Donaldov\u00FDch kriteri\u00ED jistou, kontroln\u00ED soubor bude slo\u017Een z 50-ti zdrav\u00FDch dobrovoln\u00EDk\u016F. Stanoven\u00ED hladin MMP-2,MMP-9,TIMP-2 a TIMP-9 v perifern\u00ED krvi bude provedeno metodou ELISA. Hladiny budou m\u011B\u0159eny na po\u010D\u00E1tku sledovan\u00E9ho obdob\u00ED, d\u00E1le v\u017Edy p\u0159i atace a na konci sledovan\u00E9ho obdob\u00ED. Genotypizace MMPs bude provedena metodami molekul\u00E1rn\u00ED biologie.Bude odebr\u00E1no 10 ml perifern\u00ED krve s n\u00E1slednou izolac\u00ED DNA a RNA Bude pou\u017Eita metoda PCR, restrik\u010Dn\u00ED anal\u00FDza a heteroduplexov\u00E1 anal\u00FDza.MMPs jsou slibn\u00FDm biologick\u00FDm markerem, kter\u00FD by umo\u017E\u0148oval laboratorn\u00ED monitoraci onemocn\u011Bn\u00ED a hodnocen\u00ED efektu l\u00E9\u010Dby u pacient\u016F s RS." . . "0"^^ . . . . . "NR8832" . . " MMP-9" . "Matrixmetalloproteinases in the immunopathogenesis of multiple sclerosis"@en . . "muliple sclerosis; immunopathogenesis; MMP-9; MMP-2; genotypization; MMP-2 polymorphismus; MMP-9 polymorphismus"@en . " immunopathogenesis" . "2009-12-31+01:00"^^ . . . "Byla nalezena ni\u017E\u0161\u00ED frekvence alely T -1562 genu pro MMP-9 u pacient\u016F s RS oproti zdrav\u00FDm kontrol\u00E1m. Byla prok\u00E1z\u00E1na korelace mezi t\u00ED\u017E\u00ED onemocn\u011Bn\u00ED a zv\u00FD\u0161en\u00EDm hladiny MMP-2 a sn\u00ED\u017Een\u00EDm hladiny TIMP-2."@cs .