"The proposed project is designed to obtain new information about the pathophysiological mechanisms of insulin resistance and potential for modulating the metabolic syndrome. The experiments will be carried out on non-obese models of insulin resistance hyperlipidemic rats and in transgene rats expressing FA translocase.The fatty acid transport, their utilization, storage, and DAG and LCFA-CoA will be determined. Gene expression profiling in liver, adipose tissue and muscle provide information about localization of lipid metabolism disorderes. The attention will be also focused on the possible pharmacological and pharmacogenetical aspects of the nuclear receptors agonists (PPARgamma) therapy and the implications for the storage and mobilisation of triglycerides."@en . " thiazoilidinediones" . . "2005-01-01+01:00"^^ . . "NR8495" . . . . . " protein kinase C" . "Insulin resistance; triglycerides; FA transport; FA oxidation; thiazoilidinediones; PPARgama; protein kinase C; FA translocase"@en . . "2007-12-31+01:00"^^ . "Mechanimy a d\u016Fsledky akumulace triglycerid\u016F a deriv\u00E1t\u016F mastn\u00FDch kyselin ve tk\u00E1n\u00EDch p\u0159i inzulinov\u00E9 rezistenci - mo\u017Enosti ovlivn\u011Bn\u00ED hypolipidemickou terapi\u00ED" . "V projektu budou sledov\u00E1ny patofyziologick\u00E9 mechanizmy inzulinov\u00E9 rezistence a mo\u017Enosti ovlivn\u011Bn\u00ED rezistence tk\u00E1n\u00ED agonisty PPAR gama.Pokusy budou proveden u neobezn\u00EDch model\u016F inzulinov\u00E9 rezistence a u transgenn\u00EDch potkan\u016F s exprimovanou translok\u00E1zou MK. Sledov\u00E1n bude transport MK do tukov\u00E9 a svalov\u00E9 tk\u00E1n\u011B ve vztahu k transportn\u00EDm syst\u00E9m\u016Fm MK (CD36, FABP), vliv dostupnosti glukosy na reesterifikaci a aktivita kl\u00ED\u010Dov\u00FDch enzym\u016F oxidace mastn\u00FDch kyselin. Testov\u00E1n bude vliv zv\u00FD\u0161en\u00E9 akumulace deriv\u00E1t\u016F mastn\u00FDch kyselin (diacylglycerol\u016F a mastn\u00FDch kyselin s dlouh\u00FDm \u0159et\u011Bzcem) v kostern\u00EDm svalu na zm\u011Bny v expresi a bun\u011B\u010Dn\u00E9 lokalizaci protein kinasy C, kter\u00E1 je zapojena do p\u0159enosu inzulinov\u00E9ho sign\u00E1lu. Budou sledov\u00E1ny \u00FA\u010Dinky a farmakogenetick\u00E9 aspekty l\u00E9\u010Dby inzulinov\u00E9 rezistence agonisty nukle\u00E1rn\u00EDch receptor\u016F PPARgama (thiazolidindion\u016F) ve vztahu k ukl\u00E1d\u00E1n\u00ED a mobilizaci triglycerid\u016F a genov\u00E9 expresi." . "2007-03-01+01:00"^^ . "1"^^ . . "1"^^ . " triglycerides" . "The solution has fulfilled all annotated objectives. It significantly contributed to broadening of knowledge about mechanisms of development of insulin resistance as a result of accumulation of lipid materials in livers, adipose and skeleton muscle."@en . "0"^^ . "Insulin resistance" . . . . " FA transport" . . . "http://www.isvav.cz/projectDetail.do?rowId=NR8495"^^ . . " PPARgama" . "11"^^ . . "2008-09-16+02:00"^^ . "11"^^ . . "Mechanisms and consequences of tissue triglyceride and fatty acid derivatives accumulation in insulin resistance - a possible impact of hypolipidemic therapy."@en . . "\u0158e\u0161en\u00ED splnilo v\u0161echny anotovan\u00E9 c\u00EDle. V\u00FDznamn\u00FDm zp\u016Fsobem p\u0159isp\u011Blo k prohlouben\u00ED znalost\u00ED o mechanismech rozvoje inzul\u00EDnov\u00E9 rezistence v d\u016Fsledku akumulace lipidov\u00FDch l\u00E1tek v j\u00E1trech, tukov\u00E9 tk\u00E1ni a kostern\u00EDm svalu."@cs . " FA oxidation" . .