. . "V\u00FDvoj nov\u00E9 metody kone\u010Dn\u00E9 druhov\u00E9 identifikace patogenn\u00EDch kvasinek, zalo\u017Een\u00E9 na anal\u00FDze v\u00FDsledk\u016F fingerprintingu kvasinkov\u00FDch izol\u00E1t\u016F pomoc\u00ED ode\u010Dtu teplot t\u00E1n\u00ED produkt\u016F PCR z k\u0159ivky t\u00E1n\u00ED reak\u010Dn\u00ED sm\u011Bsi po skon\u010Den\u00ED PCR. Po\u0159\u00EDzen\u00ED ve\u0159ejn\u011B p\u0159\u00EDstupn\u00E9 datab\u00E1ze v\u00FDsledk\u016F metody a v\u00FDvoj software pro usnadn\u011Bn\u00ED anal\u00FDzy v\u00FDsledk\u016F metody, oboj\u00ED umo\u017E\u0148uj\u00EDc\u00ED mezilaboratorn\u00ED srovn\u00E1n\u00ED a univerz\u00E1ln\u00ED pou\u017Eit\u00ED metody k identifikaci kvasinek v mykologick\u00FDch laborato\u0159\u00EDch. Zhodnocen\u00ED citlivosti, specifity a vypov\u00EDdac\u00ED schopnosti metody ve srovn\u00E1n\u00ED se dv\u011Bma fenotypiza\u010Dn\u00EDmi technikami (p\u0159edb\u011B\u017En\u00E1 identifikace pomoc\u00ED CHROMagaru a kone\u010Dn\u00E1 identifikace pomoc\u00ED syst\u00E9mu ID 32C) v pr\u016Fb\u011Bhu prospektivn\u00ED studie v rutinn\u00EDm provozu." . "2011-04-27+02:00"^^ . "5"^^ . . . "5"^^ . "http://www.isvav.cz/projectDetail.do?rowId=NR8365"^^ . "2009-06-30+02:00"^^ . . . " fingerprinting" . . "Druhov\u00E1 identifikace patogenn\u00EDch kvasinek zalo\u017Een\u00E1 na anal\u00FDze teploty t\u00E1n\u00ED produkt\u016F fingerprintingu" . . . "NR8365" . "0"^^ . " denaturation" . . " PCR" . . . "1"^^ . "Pathogenic yeast species identification based on analysis of melting temperature of fingerprinting products"@en . . "0"^^ . . . "2005-01-01+01:00"^^ . "McRAPD data for species identification of patogenic yeasts were stored in a public database including a processing freeware. Accurate species identification rate of an easy, rapid and economic semi-autmated McRAPD achieved 97,8%."@en . . . "Ve ve\u0159ejn\u00E9 datab\u00E1zi byla zp\u0159\u00EDstupn\u011Bna data pro McRAPD idetifikaci patogenn\u00EDch kvasinek, v\u010Detn\u011B freeware pro zpracov\u00E1n\u00ED dat. Zji\u0161t\u011Bn\u00E1 m\u00EDra p\u0159esnosti druhov\u00E9 identifikace snadn\u00FDm rychl\u00EDm a ekonomick\u00FDm poloautomatick\u00FDm postupem Mc RAPD dos\u00E1hla 97,8%."@cs . " RAPD" . . " Candida" . " yeasts" . "identification" . . "identification; yeasts; Candida; fingerprinting; PCR; RAPD; denaturation; LightCycler"@en . "Development of a new approach to ultimate pathogenic yeast species identification based on melting curve analysis of PCR-fingerprinting products. Establishment of a public database of results obtained with the new method. Development of a software enabling user-friendly analysis and interlaboratory reproducibility of the results and thus allowing universal applicability of the method in mycology. Prospective study to evaluate sensitivity, specifity and predictive value of the method in routine use compared to 2 phenotyping techniques (presumptive identification using CHROMagar and ultimate identification using ID 32C)."@en . .