"Transplantace ostr\u016Fvk\u016F p\u0159ipraven\u00FDch in vitro z kmenov\u00FDch bun\u011Bk dosp\u011Bl\u00E9ho pankreatu" . . . "The study evaluated the differetion of the Langerhans cells in vitro. It is an important step for the modern directions in clinical transplantion of pancreas."@en . . . . "1"^^ . " transplantation of beta cells" . . . "0"^^ . "2"^^ . . " diabetes" . " insulin" . "2"^^ . "0"^^ . "stem cells" . . "http://www.isvav.cz/projectDetail.do?rowId=NR8031"^^ . . . . . "C\u00EDlem experimentu je navozen\u00ED proliferace a diferenciace izolovan\u00FDch kmenov\u00FDch bun\u011Bk z dosp\u011Bl\u00E9ho pankreatu potkan\u016F a \u010Dlov\u011Bka sm\u011Brem k fenotypu beta bun\u011Bk. Kvalita bun\u011Bk bude testov\u00E1na expres\u00ED gen\u016F inzul\u00EDnu, glukagonu a transkrip\u010Dn\u00EDch faktor\u016F (PDX-1, PAX-4). Schopnost produkovat inzul\u00EDn bude me\u0159ena v prvn\u00ED f\u00E1zi ve stimula\u010Dn\u00EDch kultur\u00E1ch a ve druh\u00E9 f\u00E1zi transplantac\u00ED diabetick\u00FDm zv\u00ED\u0159at\u016Fm. Epiteloidn\u00ED struktury podobn\u00E9 pankreatick\u00FDm ostr\u016Fvk\u016Fm mohou p\u0159edstavovat n\u00E1hradn\u00ED zdroj inzul\u00EDn produkuj\u00EDc\u00ED tk\u00E1n\u011B k transplantaci" . . "2007-08-08+02:00"^^ . . "The aim of the project is to induce the proliferation of stem cells isolated from adult rat and human pancreases and to enable them to differentiate towards the phenotype of pancreatic cells. The cell quality will be tested by means of expression of genes for insulin, glucagon and characteristic transcription factors (PDX-1, PAX-4). The ability to produce insulin will be measured in the first phase using stimulation cultures and in the second phase by means of transplantation to diabetic animals. Aggregates of epitheloid cells similar to pancreatic islets may represent an artificial source of insulin producing tissue for transplantation."@en . . "Transplantation of islets generated in vitro from stem cells of adult pancreas"@en . . "Studie p\u0159isp\u011Bla k pozn\u00E1n\u00ED diferenciace Langerhansov\u00FDch bun\u011Bk in vitro s v\u00FDznamem pro klinick\u00E9 vyu\u017Eit\u00ED u transplantacce."@cs . . "stem cells; diabetes; transplantation of beta cells; pancreatic islets; insulin; feeder"@en . . " pancreatic islets" . . "NR8031" .