"PCR metoda ned\u00E1v\u00E1 v diagnostice infekce Pseudomonas aeruginosadostate\u010Dn\u011B spolehliv\u00E9 v\u00FDsledky a proto ji nelze zat\u00EDm doporu\u010Dit k rutin\u00EDmu pou\u017Eit\u00ED pro z\u00E1chyt t\u00E9to infekce a stanoven\u00ED l\u00E9\u010Dby."@cs . . . . "2007-08-08+02:00"^^ . . . " PCR" . . "Pseudomonas aeruginosa; Cystic fibrosis; antibiotic; treatment; PCR; detection; fingerprinting"@en . . . " antibiotic" . . . . . " treatment" . "The effective treatment of infection with Pseudomonas aeruginosa in patients with cystic fibrosis requires early pathogen detection. Highly sensitive detection of the P.aeruginosa using nested PCR will be the indication to start an antibiotic therapy. The benefit from novel therapeutic protocol will be evaluated by comparison with clinical and microbiological data of historical controls (2001-2004). Their sputa will be retrospectively examined for the presence of P.aeruginosa using novel PCR. The potential occurrence of transmissible strain of P.aeruginosa in a cohort of our patients with cystic fibrosis will be checked using two genotyping techniques: RAPD and macrorestriction typing with subsequent PFGE."@en . . . "Pseudomonas aeruginosa" . "NR7974" . " detection" . . "Efektivn\u00ED l\u00E9\u010Dba infekce Pseudomonas aeruginosa u pacient\u016F s cystickou fibr\u00F3zou vy\u017Eaduje jej\u00ED \u010Dasnou diagnostiku. Velmi citliv\u00FD p\u0159\u00EDm\u00FD pr\u016Fkaz patogenu pomoc\u00ED nested PCR bude indikac\u00ED k zah\u00E1jen\u00ED antibiotick\u00E9 l\u00E9\u010Dby. Prosp\u011Bch z nov\u00E9ho l\u00E9\u010Debn\u00E9ho protokolu bude stanoven porovn\u00E1n\u00EDm s klinick\u00FDmi daty historick\u00FDch kontrol (2001 a\u017E 2004) a s jejich mikrobiologick\u00FDmi v\u00FDsledky, je\u017E budou dopln\u011Bn\u00E9 o retrospektivn\u00ED PCR vy\u0161et\u0159en\u00ED sputa na p\u0159\u00EDtomnost P.aeruginosa. Existence p\u0159enosn\u00E9ho kmene P.aeruginosa v komunit\u011B pacient\u016F s cystickou fibr\u00F3zou bude zji\u0161\u0165ov\u00E1na pomoc\u00ED dvou genotypiza\u010Dn\u00EDch technik: RAPD a makrorestrik\u010Dn\u00ED typizace s vyu\u017Eit\u00EDm PFGE." . . "Pseudomonas aeruginosa u pacient\u016F s cystickou fibr\u00F3zou: vliv \u010Dasn\u00E9 PCR-indikovan\u00E9 terapie, sledov\u00E1n\u00ED cest \u0161\u00ED\u0159en\u00ED infekce" . "http://www.isvav.cz/projectDetail.do?rowId=NR7974"^^ . "1"^^ . . "1"^^ . "Pseudomonas aeruginosa in patients with cystic fibrosis: an impact of early PCR-indicated therapy, identification of routes of the infection spread"@en . "1"^^ . . . "1"^^ . " Cystic fibrosis" . "0"^^ . "The PCR method does not reliakly detect Pseudomonas aeruginosa and there is not recommended for rutine identification of this infection and planning a therapentic strategy for cystic fibrosis patients."@en .