"7"^^ . " Ductal cells" . "7"^^ . . . . . "Byly z\u00EDsk\u00E1ny nov\u00E9 poznatky o expresi gen\u016F souvisej\u00EDc\u00EDch s progres\u00ED dukt\u00E1ln\u00EDch a lobul\u00E1rn\u00EDch karcinom\u016F mamy.V\u00FDsledky naleznou uplatn\u011Bn\u00ED v diagnostice a klasifikaci karcinomu prsu a mohly by poslou\u017Eit i p\u0159i v\u00FDvoji c\u00EDlen\u00E9 terapie"@cs . " Lobular cells" . "Breast; Cancer; Ductal cells; Lobular cells; Expression profile; Diagnostics"@en . "Anal\u00FDza genov\u00E9ho expresn\u00EDho profilu norm\u00E1ln\u00EDch dukt\u00E1ln\u00EDch a lobul\u00E1rn\u00EDch lumin\u00E1ln\u00EDch bun\u011Bk a jeho vztah k histopatologick\u00E9 klasifikaci dukt\u00E1ln\u00EDch a lobul\u00E1rn\u00EDch karcinom\u016F prsu" . . . "Nal\u00E9zt typick\u00E9 molekul\u00E1rn\u00ED znaky, kter\u00E9 mohou pomoci rozli\u0161it mamm\u00E1rn\u00ED karcinomy dukt\u00E1ln\u00EDho nebo lobul\u00E1rn\u00EDho typu. Analyzovat expresn\u00ED profily norm\u00E1ln\u00EDch lumin\u00E1ln\u00EDch bun\u011Bk dukt\u00E1ln\u00EDho a lobul\u00E1rn\u00EDho typu, nal\u00E9zt molekul\u00E1rn\u00ED znaky, kter\u00E9 tyto bun\u011B\u010Dn\u00E9 typy charakterizuj\u00ED a srovnat jejich v\u00FDskyt v typick\u00FDch morfologick\u00FDch entit\u00E1ch dukt\u00E1ln\u00EDho a lobul\u00E1rn\u00EDho karcinomu. Metodicky pou\u017E\u00EDt mikrodisekci bun\u011Bk ze zmra\u017Een\u00FDch \u0159ez\u016F, extrakci RNA z disekovan\u00FDch bun\u011Bk, %22high-fidelity%22 amplifikaci mRNA, mikro\u010Dipovou anal\u00FDzu expresn\u00EDch zm\u011Bn a imunohistochemickou nebo hybridiza\u010Dn\u00ED anal\u00FDzu genov\u00E9 exprese. Projekt m\u016F\u017Ee b\u00FDt d\u016Fle\u017Eit\u00FD pro pochopen\u00ED klinick\u00E9ho pr\u016Fb\u011Bhu onemocn\u011Bn\u00ED, tak i pro zlep\u0161en\u00ED jeho diagnostiky. D\u00E1le by mohly b\u00FDt z\u00EDsk\u00E1ny nov\u00E9 poznatky o patomorfologii mamm\u00E1rn\u00EDch karcinom\u016F i o molekul\u00E1rn\u00EDch mechanismech jejich vzniku a v\u00FDvoje." . "NR7844" . . . . " Cancer" . "The analysis of gene expression profile of normal ductal and lobular luminal cells and its relevance to the histopathological classification of ductal and lobular breast carcinomas"@en . . . "Breast" . . "New findings on gene expression of invasive ductual and lobular breast cancer related to tumor progression werw obtained. The results will possible tu use for cancer diagnostics and classification and potentially for targeted therapy."@en . . . "To find typical molecular markers, which could identify breast carcinomas of either ductal or lobular origin. To analyse the expression profiles of normal mammary ductal and lobular luminal cells, to find molecular markers that characterise these cell types and to compare their expression with that of the typical morphological entities of ductal and lobular carc To use the microdissection and RNA extraction from microdissected cell samples, high-fidelity mRNA amplification, microarray analysis, and immunohistochemical or in situ hybridisation analysis of gene expression. This comparison should have a significant clinical and diagnostical implications. In addition, the solution of project can bring a new knowledge on pathomorphology of breast cancer and on molecular mechanisms of breast cancer development."@en . "2007-08-08+02:00"^^ . . "http://www.isvav.cz/projectDetail.do?rowId=NR7844"^^ . . . "0"^^ . " Expression profile" . "0"^^ . . . "1"^^ . . .