. "Mezi nej\u010Dast\u011Bj\u0161\u00ED genetick\u00E9 alterace spojen\u00E9 se vznikem glioblastom\u016F pat\u0159\u00ED mutace a delece n\u00E1dorov\u00FDch supresor\u016F PTEN, p53 a amplifikace receptor\u016F pro epiderm\u00E1ln\u00ED r\u016Fstov\u00FD faktor (EGFR) a trombocyt\u00E1rn\u00ED r\u016Fstov\u00FD faktor (PDGFR). C\u00EDlem projektu je 1) ov\u011B\u0159en\u00ED vlivu PTEN na inhibici proliferace gli\u00E1ln\u00EDch bun\u011B\u010Dn\u00FDch lini\u00ED, 2) anal\u00FDza vlivu PTEN na expresi vybran\u00FDch protein\u016F reguluj\u00EDc\u00EDch dr\u00E1hu PI3-K/PKB/Akt, MAPK, bun\u011B\u010Dn\u00FD cyklus a regul\u00E1tor angiogeneze trombospondin 1. 3) anal\u00FDza vztahu mezi expres\u00ED PTEN a protein\u016F reguluj\u00EDc\u00EDch PI3-K/PKB/Akt, MAPK dr\u00E1hu, bun\u011B\u010Dn\u00FD cyklus a trombospondin 1 u souboru bioptick\u00FDch vzork\u016F pacient\u016F s low grade a high grade astrogli\u00E1ln\u00EDmi n\u00E1dory. Pro experimenty pl\u00E1nujeme pou\u017E\u00EDt metody kultivace bun\u011B\u010Dn\u00FDch lini\u00ED, transfekce DNA, detekce bun\u011B\u010Dn\u00E9 proliferace, elektrofor\u00E9zu protein\u016F, %22Western blot%22 anal\u00FDzu, imunocytochemickou a imunohistochemickou detekci." . . . " Protein-phosphatase" . "2007-08-08+02:00"^^ . "http://www.isvav.cz/projectDetail.do?rowId=NR7828"^^ . "PI3-K/PKB/Akt pathway; Cell cycle; PTEN; Protein detections; Glioblastomas; Cell lines; Protein-phosphatase; Tumour suppressor"@en . "0"^^ . . "PI3-K/PKB/Akt pathway" . "The suppressor gene PTEN plays a role in cell cycle regulation in glioblastoma cell lines."@en . "4"^^ . . " Protein detections" . " Cell lines" . "4"^^ . . "1"^^ . . "1"^^ . . . " Cell cycle" . . . " Glioblastomas" . . "NR7828" . . "Supresorov\u00FD gen PTEN hraje v\u00FDznamnou roli v regulaci bun\u011B\u010Dn\u00E9ho cyklu v bun\u011B\u010Dn\u00E9 linii glioblastomu."@cs . . . . . "Studium v\u00FDznamu protein\u016F reguluj\u00EDc\u00EDch sign\u00E1ln\u00ED dr\u00E1hu PI3-K/PKB/Akt a bun\u011B\u010Dn\u00FD cyklus pro patogenezi glioblastom\u016F" . . . " PTEN" . "The most frequent alterations in glioblastomas are mutations and deletions of the tumour suppressor genes PTEN and p53 and amplification of epidermal growth factor receptor (EGFR) and platelet derived growth factor receptor (PDGFR). The aim of the project is 1)verification of PTEN influence on the inhibition of glioma cell lines proliferation, 2)analysis of PTEN influence on the expression of proteins regulating PI3-K/PKB/Akt, MAPK pathways, cell cycle and thrombpspondin 1. 3)analysis of the relationship between PTEN expression and expression of proteins regulating PI3-K/PKB/Akt, MAPK pathways, cell cycle and thrombospondin 1 in the collection of samples from low grade and high grade astroglial tumours. For experiments we plan to use these methods: cell culture cultivation, transfection of DNA, detection of cell proliferation, protein electrophoresis, Western blot analysis, immunocytochemistry and immunohistochemistry."@en . "Study of importance of proteins regulating PI3-K/PKB/Akt signaling pathway and cell cycle for glioblastoma pathogenesis"@en . . .